National Research Council of Canada

Ramesh B Nair

Pierre Hucl

Wilfred A Keller

Gopalan Selvaraj

Raju S Datla

Ravindra N Chibbar

Monica Baga

Graham J Scoles

Mingsheng Peng

Richard W Joy, Iv

Anne Repellin

Ming Gao

The invention provides a novel starch branching enzyme that is bound to A-type starch granules in wheat, barley, rye or triticale. The enzyme is not substantially associated with B-type starch granules. A cDNA sequence encoding an isoform of the enzyme has been isolated from the wheat cultivar Fielder and deduced amino acid sequence has been determined.

Starch branching enzymes

The sinapine content of seeds of , and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H:) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.

Transgenic reduction of sinapine in crucifera

Growing community of inventors

Saskatoon, Canada

Ramesh B Nair