The General Hospital Corporation

Whitehead Institute for Biomedical Research

Massachusetts Institute of Technology

Siemens Medical Solutions Usa, Inc.

The Brigham and Women's Hospital, Inc.

Dana-farber-cancer Institute Inc.

Csl Behring Gmbh

Ralph Weissleder

Hakho Lee

Alexei A Bogdanov

Scott A Hilderbrand

Lee Josephson

Edmund J Keliher

Cesar Castro

Umar Mahmood

Thomas Reiner

Hyungsoon Im

David Issadore

Neal K Devaraj

Ching-Hsuan Tung

Vasilis Ntziachristos

Kimberly A Kelly

Nabeel Bardeesy

Mark R Karver

Tae-Jong Yoon

Sarit S Agasti

Hidde L Ploegh

J Manuel Perez

Thomas J Brady

Rahul Sheth

Peter Panizzi

Mohammad Rashidian

Vanessa M Peterson

Rabi Upadhyay

Adeeti Ullal

Jason R McCarthy

Michael John Cima

Arne Hengerer

Gerhard Dickneite

Marc Nolte

Ronald A DePinho

Pallab Banerjee

Yi Sun

James P Basilion

Hsing-Ying Lin

Jonathan Axel Carlson

Jamil Azzi

Matthias P Nahrendorf

John W Chen

Xin Lu

Guido Stoll

Sangmoo Jeong

Ghyslain Budin

Jongmin Park

Hyun Jung Chung

Balazs R Varga

Kyungheon Lee

Jun Song

Rui Hong

Fangwei Shao

Manel Querol

Bogdan Von Rueckmann

Ralph Gareus

Wilhelm Dürr

Wilfried Reichardt

Ennio Antonio Chiocca

Labros Meimetis

Ronald Callahan

Matthias Nahrendorf

Alexei Bogdanov

Kimberly Kelly

Kisoo Park

Chen-han Huang

The present invention, in some aspects, provides methods, reagents, compositions, and kits for the radiolabeling of proteins, for example, of proteins useful for positron emission tomography (PET) or single-photon emission computed tomography (SPECT) (e.g., for diagnostic and therapeutic applications), using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a radioactive agent or molecule to diagnostic or therapeutic peptides or proteins. Compositions comprising sortagged, radiolabeled proteins as well as reagents for generating radiolabeled proteins are also provided. Kits comprising reagents useful for the generation of radiolabeled proteins are provided, as are precursor proteins that comprise a sortase recognition motif.

18F labeling of proteins using sortases

The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.

Portable diffraction-based imaging and diagnostic systems and methods

The present application relates to functionalized 1,2,4,5-tetrazine compounds. The compounds are useful in compositions and methods using bioorthogonal inverse electron demand Diels-Alder cycloaddition reactions for the rapid and specific covalent delivery of a 'payload' to a ligand bound to a biological target.

Functionalized 1,2,4,5-tetrazine compounds for use in bioorthogonal coupling reactions

Target analyte detection devices include a housing having a potentiostat and a microcontroller coupled to the potentiostat. The device also includes a substrate having a plurality of electrodes on a first surface of the substrate. A first set of electrodes of the plurality of electrodes defines a first sample detection region. The substrate is removably attachable to the housing such that the first set of electrodes is coupled to the potentiostat upon attaching the substrate to the housing. The device also includes a magnet assembly couplable to a second surface of the substrate. The magnet assembly includes a magnet positioned in the magnet assembly such that a magnetic field from the magnet extends through the substrate and the first set of electrodes into an area above the first sample detection region upon coupling the magnet assembly to the substrate.

Magnetic electrochemical sensing

This invention relates to methods for diagnosing cancer, e.g., cancer of epithelial origin, by detecting the presence of tumor cells in a sample, based (at least in some embodiments) on the quantification of levels of four biomarkers, MUC1, EGFR, EpCAM, and HER2. In some embodiments, the methods are performed using diagnostic magnetic resonance (DMR), e.g., with a portable relaxometer or MR imager.

Molecular analysis of tumor samples

This document relates to compounds useful for targeting PARP1. Also provided herein are methods for using such compounds to detect and image cancer cells.

Compositions and methods for in vivo imaging

Described herein are methods, compositions, kits and systems for multiplexed detection of target molecules from a sample. In some embodiments, the methods, compositions, kits and systems can be used to perform multiplexed protein analysis of a sample (e.g., a sample comprising a small number of cells or a single-cell sample). In some embodiments, the same sample subjected to a multiplexed protein analysis using the methods, compositions, kits and systems described herein can also be subjected to a nucleic acid (e.g., RNAs, microRNAs, and/or DNA) analysis, thereby creating an integrated expression profiling from a limited amount of sample.

Methods, kits, and systems for multiplexed detection of target molecules and uses thereof

The present invention, in some aspects, provides methods, reagents, compositions, and kits for the radiolabeling of proteins, for example, of proteins useful for positron emission tomography (PET) or single-photon emission computed tomography (SPECT) (e.g., for diagnostic and therapeutic applications), using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a radioactive agent or molecule to diagnostic or therapeutic peptides or proteins. Compositions comprising sortagged, radiolabeled proteins as well as reagents for generating radiolalebed proteins are also provided. Kits comprising reagents useful for the generation of radiolabeled proteins are provided, as are precursor proteins that comprise a sortase recognition motif.

F labeling of proteins using sortases

Disclosed herein are compositions and methods for exosome detection with high sensitivity by using a nano-plasmonic sensor. The nano-plasmonic sensor comprises a plurality of nanoapertures suitable for transmission measurements. The detection sensitivity is on the order of 10-fold higher than western blotting and 10-fold higher than enzyme-linked immunosorbent assay (ELISA). A portable imaging system is also disclosed, enabling rapid and high-throughput detection of exosomes. The nano-plasmonic sensor and imaging system can be useful in diagnostics.

Nano-plasmonic sensor for exosome detection

This present application relates to fluorescent tetrazine-containing compounds consisting of a single pi-system. For example, a compound of Formula (I): or a salt thereof, wherein: F is a fluorophore, L is a conjugated linker, and Tz is a substituted or unsubstituted tetrazine; wherein the linker bridges the Tz and F moieties in a single conjugated pi-system. Also provided herein are methods of using the compounds provided herein for biomedical imaging.

Bioorthogonal turn-on probes

The present invention provides methods and compositions based on optical sensor conjugates that are useful for detecting reactive oxygen, reactive nitrogen, or both species that are a direct result of inflammation caused by tissue damage.

Optical sensor conjugates for detecting reactive oxygen and/or reactive nitrogen species in vivo

The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.

Magnetic labeling of bacteria

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Peabody, MA, United States of America

Ralph Weissleder