Carl Zeiss Microscopy Gmbh

Ralf Steinmeyer

Peter Schoen

Reiner Mitzkus

Matthias Langhorst

Cornelia Bendlin

Timo Rojahn

Thorsten Kues

Klaus Becker

Peter Schön

Maik Sommer

The invention relates to a method for recording images with a light microscope, wherein a specimen container with a specimen is arranged on a specimen holder of the light microscope, and wherein illuminating light is guided onto the specimen. The illuminating light can hereby be cut in a cross-section transversely to an optical axis of the light microscope through a wall of the specimen container to a limited cross-sectional region. First and second diaphragm settings are determined and set, for the limited cross-sectional region of the illuminating light defined by the wall of the specimen container, in which the diaphragm covers equal sized portions of the limited cross-sectional region. In addition the invention relates to a light microscope which is adapted in particular to carry out the method.

Light microscope and method for recording images with a light microscope

A procedure for the correction of spherical aberration in microscopic applications, wherein various recordings of a specimen to be observed are taken and evaluated for the purpose of changing the setting values of the optical system. The correction values are stored in a correction matrix as a function of the recording position, the recording time, the wavelength, and the temperature, wherein the determination and storage of the correction values are carried out in each recording position in the x, y, and z coordinates, and/or the correction values are determined after a selection of grid points by interpolation, so that the correction values of the interpolated correction matrix are the starting values for the subsequent exact determination by measurement.

Method for the correction of spherical aberration in microscopic applications

A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.

Method for detecting and controlling supply of an immersion medium

A transmitted-light microscope for imaging well-shaped, liquid-containing sample vessels, wherein the transmitted-light microscope has an illumination beam path for illuminating the sample vessel with an illumination beam bundle, from above, along an optical axis, wherein the illumination beam path has an illuminating element aligned to the optical axis, which element irradiates the illumination beam bundle onto the sample vessel, an imaging beam path for imaging the sample vessel from below along the optical axis and a pipette access channel for introducing a reagent into the sample vessel, wherein the illuminating element is annular and has an opening on the optical axis through which opening runs the pipette access channel.

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Hannover, Germany

Ralf Steinmeyer