Boehringer Mannheim Gmbh

Other

Sudzucker Aktiengesellschaft Mannheim/ochsenfurt

Sudzucker Aktiengesellschaft

Basf Aktiengesellschaft

Degussa Aktiengesellschaft

Roche Diagnostics Gmbh

Universitãt Stuttgart

University of Stuttgart

Evonik Degussa Gmbh

Ralf Mattes

Markwart Kunz

Kathrin Klein

Christoph Syldatk

Burkard Wilms

Anja Wiese

Stephan Fischer

Christoph Kessler

Rudolf Seibl

Josef Altenbuchner

Mohammed Munir

Bernhard Hauer

Rainer Rudolph

Thomas Friedrich

Andreas Bommarius

Mohammad Munir

Wilhelm Tischer

Hubert Schiweck

Gudrun Schmitz

Markus Pietzsch

Hurbert Schiweck

Ulrich Brinkmann

Hans-Joachim Holtke

Hans Robert Scholer

Helmut Lenz

Klaus Beaucamp

Peter Buckel

Werner Stock

Maria Kesseler

Marion Ress-Löschke

Dirk Engels

Klaus Dieter Kulbe

Joseph Altenbuchner

Hermann Graf

Hans J Holtke

Sabine Stegmaier

A whole cell catalyst is described comprising a hydantoinase, a racemase and a carbamoylase. Thus this catalyst is able to degrade hydantoins directly into the amino acids. Additionally, a process for the production of this catalysts and for the production of amino acids is claimed.

Whole cell catalyst

This invention concerns a method for enzymatic preparation of 6-O-&#x3b1;-D-glucopyranosyl-D-sorbitol (1,6-GPS) from isomaltulose. It is specified that the isomaltulose is put into an aqueous reaction solution containing a unit having sorbitol dehydrogenase (SDH) activity, incubated, and then 1,6-GPS is recovered from the reaction solution.

Method for producing 6-0-&#x3b1;-D-glucopyranosyl-D-sorbitol

The invention relates to sucrose isomerases, to DNA sequences that code for sucrose isomerases, and to novel processes for the production of non-cariogenic sugars.

Preparation of acariogenic sugar substitutes

The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

L-pantolactone-hydrolase and a method for producing D-pantolactone

A novel rec-L-N-carbamoylase fromand its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.

Recombinant L-N-carbamoylase from Arthrobacter aurescens and method of producing L-amino acids therewith

The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

Method for producing chiral carboxylic acids from nitriles with the assistance of a nitrilase or microoganisms which contain a gene for the nitrilase

A microorganism is described which is transformed with DNAs which encode a hydantoinnase, a racemase, and a carbamoylase. As a result, the microorganism is able to degrade hydantoins directly to amino acids. A process for the production of the microorganism and a process for producing amino acids with the microorganism is also described.

Whole cell catalysts

A novel rec-L-N-carbamoylase from and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.

The present invention provides a process for the expression of a recombinant gene, which contains AGA and/or AGG codons for arginine, in,after transformation with an expression vector which contains the recombinant gene, wherein the amount of t-RNA present in the,cells which incorporates arginine and recognises the codons AGG and AGA is increased to at least fivefold of the amount normally occurring in these cells.

Process for producing recombinant proteins using a gene for tRNA

The invention relates to sucrose isomerases, to DNA sequences coding therefor, and to novel processes for the production of non-cariogenic sugars.

The invention relates to sucrose isomerases, to DNA sequences coding therefor, and to novel processes for producing noncariogenic sugars.

Sucrose metabolism mutants

DNA's encoding sucrose isomerase and palatinase

For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.

Process for the detection of nucleic acids

A process for the activation of disulphide linked recombinant proteins expressed in prokaryotes is described. The process includes cell digestion, solubilization under denaturing and reducing conditions and activation under oxidizing conditions in the presence of GSH/GSSG and a non-denaturing amount of a denaturing agent.

Process for the activating of gene-technologically produced,

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Stuttgart, Germany

Ralf Mattes