Epigenomics Ag

Therawis Diagnostics Gmbh

Ralf Lesche

Fabian Model

Cathy Lofton-Day

Andrew Sledziewski

Matthias Schuster

Reimo Tetzner

Xiaoling Song

Thomas Hildmann

Juergen Distler

Catherine E Lofton-Day

Andrew Z Sledziewski

Sabine Maier

Peter Adorjan

Manfred Schmitt

Dimo Dietrich

Nadia Harbeck

Susan Cottrell

John W Martens

Heinz Hoefler

John Foekens

Thomas Koenig

Anne Fassbender

Matthias Burger

Kurt Berlin

Oliver Hartmann

Christian Piepenbrock

Manuel Krispin

Jürgen Distler

Antje Kluth Lukas

Inko Nimmrich

Almuth Marx

Tamas Rujan

Antje Kluth

Theo De Vos

Ina Schwope

Volkmar Mueller

Suzanne Mooney

Achim Plum

Gunter Weiss

Frederique Spyratos

Vincent Vuaroqueaux

Serenella Eppenberger-Castori

Joern Dietrich

Volkmar c/o Department Of Gynecology Müller

Frédérique Spyratos

John Martens

Jörn Dietrich

Heinz Höfler

Thomas König

The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

Methods and nucleic acids for analyses of cellular proliferative disorders

The present invention relates to methods for predicting the outcome of anthracycline treatment of cell proliferative disorder patients. This is achieved by determining the expression level of at least one gene selected from the group consisting of PITX2; TFF1 and PLAU. The invention also relates to sequences, oligonucleotides and antibodies which can be used within the described methods.

Markers for the prediction of outcome of anthracycline treatment

Provided are methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. Further provided are novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said genes as well as methods, assays and kits thereof.

Method and nucleic acids for the analysis of colon cell proliferative disorders

Methods and nucleic acids for the analyses of cellular proliferative disorders

The invention provides methods, nucleic acids and kits for determining the prognosis of a subject having cell proliferative disorder, preferably cancer. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.

Methods and nucleic acids for analyses of cell proliferative disorders

The present invention relates to modified and genomic sequences, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA, as well as to a method for predicting the disease free survival and/or response of a subject with a cell proliferative disorder of the breast tissues, to endocrine treatment.

Methods for the prognosis of breast cancer

Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders as well as therapy thereof by analysis of the gene EYA4 and its promoter and regulatory sequences. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said gene as well as methods, assays and kits thereof.

Method and nucleic acids for the analysis of colorectal cell proliferative disorders

Methods and nucleic acids for analysis of cellular proliferative disorders

Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.

Method for determining the methylation pattern of a polynucleic acid

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Berlin, Germany

Ralf Lesche