Leica Biosystems Nussloch Gmbh

Ralf Eckert

Hermann Ulbrich

Stella Louise Knorr

Andrew Guy

Markus Berberich

Fernando Dias

Chris Ryan

Neil Sanut

Fiona Tarbet

Michael Eberhard

Florian Böhl

Karin Flieger

Graeme Robertson

Uwe Höfling

The invention relates to a method for embedding a tissue sample into an embedding medium. In a first step the tissue sample is held in an intended orientation in a container by means of a holding element that presses the tissue sample against a base of the container. In a further step a liquid embedding medium that has a temperature above 64 degrees Celsius, in particular in the range from 65 degrees Celsius to 67 degrees Celsius, or a temperature of 66 degrees Celsius, is poured into the container, and the base of the container is cooled. In a further step a separating motion is executed by way of which the tissue sample and the holding element move away from each other, those layers of the embedding medium through which the holding element moves during the separating motion having, during the separating motion, temperatures in the range from 54 degrees Celsius to 64 degrees Celsius, in particular of 60 degrees Celsius, and/or those portions of the embedding medium which are directly adjacent to the holding element having, during the separating motion, a temperature in the range from 54 degrees Celsius to 64 degrees Celsius, in particular of 60 degrees Celsius.

Method and automatic machine for embedding a tissue sample into an embedding medium

An apparatus for holding a tissue sample including a retaining member having a first tissue engaging surface and at least one biasing element, the first tissue engaging surface being moveably attached to the retaining member by said biasing element; and a base having a second tissue engaging surface and configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other, wherein the at least one biasing element urges the first tissue engaging surface toward the second tissue engaging surface to retain the tissue sample therebetween in the interior area.

Tissue cassette with biasing element

The invention relates to a melting apparatus () for melting paraffin (), having: a melting container () for receiving paraffin () to be melted; a storage container () for storing molten paraffin (); having a melting container heating device () for heating the melting container (), having a storage container heating device () for heating the storage container (), having a fluid connection () fluidically connecting the melting container () and the storage container; the melting container (), the storage container (), and the fluid connection () being arranged so that molten paraffin () flows out of the melting container () into the storage container ().

Melting apparatus for metered melting of paraffin

The disclosure relates to a processor for processing histological samples. The processor comprises an inlet for insertion of a closed fixing container having the samples present therein.

Processor for processing histological samples

The invention relates, in accordance with one aspect, to a portable device for transporting at least one histological sample or at least one cassette containing a histological sample. The device is attachable to a processing station for processing 5 histological samples. In the attached state, energy for cooling the histological sample is transferred from the processing station to the device or vice versa. In accordance with another aspect of the invention, a processing station for processing a histological sample is provided, which station is embodied for releasable attachment of a portable device. The processing station is further embodied to transfer energyfor cooling to, or receive it from, an attached device.

Portable device for transporting a histological sample

The invention relates to a processor for working on histological samples. The processor has an inlet for the insertion of a fixing container, particularly a closed fixing container, with the samples located in the latter.

Processor for working on histological samples

A method for treating a tissue sample including placing at least one tissue sample on an cassette which has: a retaining member, a base and at least one biasing element; placing the at least one tissue sample in the tissue cassette; attaching the base and the retaining member to retain the tissue sample; processing the tissue sample in the tissue cassette with one or more solvents; and embedding the tissue sample in a paraffin to form an portion of paraffin in which the tissue sample is embedded in the tissue cassette, wherein the embedding comprises adding molten paraffin to the interior area of the tissue cassette and allowing the paraffin to become solid.

Method for processing and embedding tissue

A freezing microtome for the production of microscopable thin sections of tissue samples (), comprising a cooling device () for freezing the tissue samples (), a cutting device for cutting the frozen tissue samples () and a working chamber, in which the cutting device and at least a part of the cooling device () are arranged is described. The part of the cooling device () arranged in the working chamber contains a liquid coolant (), in which the tissue samples () can be inserted for freezing.

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Schriesheim, Germany

Ralf Eckert