Miles Inc.

Miles Laboratories, Inc.

Raleigh A Carmen

Barry S Leng

Edward J Nelson

Chiyong Chong

Willie J Lewis

Chi-Yong Chong

Eva Sajan

A closed system for inactivating viruses in blood or blood components. The system includes at least one plastic bag which communicates with a separate container including a single viricidal substance or separate substances which, when mixed, provide in situ generation of a viricidal substance. The viricidal substance can be transferred into the bag for subsequent viricidal use on blood components or for use on components in the bag. A preferred system provides a means for the in-situ generation of ClO.sub.2 from sterilizable, separate substances for viricidal use on blood or blood components.

Method for inactivating viruses in blood using chlorine dioxide

Platelet concentrate containing less then 10.sup.6 white blood cells and prepared from a unit of whole blood sample in a closed multiple blood bag system within less than about eight hours after the whole blood is removed from a human. The concentrate is contained in a platelet storage bag made from polyvinyl chloride plasticized with either trioctyltrimellitate or ethylene vinyl acetate.

Platelets concentrate with low white blood cells content

Blood bag system for separating whole blood into upper, middle, and lower components which can then be isolated from each other in an automated manner. The system comprises a primary or main blood bag having first and second outlet ports. The first outlet port communicates via tubing with a first satellite bag. The second outlet port communicates via tubing with a second satellite bag at one end and at the other end communicates with a tubular member extending into and toward the bottom of the main bag. In use whole blood is drawn into the main bag through an inlet port and centrifuged to form an upper, less dense plasma portion, a lower, more dense red blood cell (RBC) portion and an intermediate portion of buffy coat (including platelets). Pressure is then applied to the bag with the first outlet open and the second outlet closed to express all of the upper plasma through the first outlet port after which the first outlet port is closed and the second outlet is opened. The lower RBCs are expressed by pressure through the tubular member and second outlet, leaving only the intermediate buffy coat in the main bag. The system may be automated with sensors to monitor and control the flow of fluids through each outlet and from the main bag.

Blood separation system

Filtration of white blood cells (WBCs) directly from platelet rich plasma (PRP) rather than from subsequently made platelet concentrates (PC). Earlier filtration is effective at removing most WBCs with minimal platelet loss and permits storage of platelets for at least 5 days. The filtration is preferably done with a closed, ready-to-use system comprising a WBC filter disposed between a first blood bag and at least two empty secondary bags. A fourth bag may be included in the closed system to contain a platelet additive solution. System can remove at least 95% of the WBCs with less than 10% loss in platelets. In very preferred embodiments at least 99.5% of the original WBCs are removed by filtration prior to platelet storage.

Pre-storage filtration of platelets

An improved heat sterilizable blood bag system adapted for containing and mixing of different materials originally placed in separate but potentially communicating compartments. The improvement comprises of having a smaller squeezable compartment for one material attached to and capable of communicating with a larger compartment for the other material. The material in the smaller compartment is separated from the interior of the larger compartment by means of an externally manipulatable closure means which, when opened, permits mixture of the contents in the first compartment and contents of the second compartment when the smaller compartment is repeatedly squeezed.

Sterilizable system for blood storage

A generally flat, elongated edge-sealed polymeric blood bag having a length to width ratio of at least 2 to 1 and one end portion in tapering communication with a connected tubing. When the bag is filled with a mixture of blood components which are then separated, the tapering end portion expands to form a funnel-like guide for directing a separated component from the bag through the tubing in a substantially unobstructed manner. Bag is especially useful for separating and isolating the components of a neocyte/gerocyte red blood cell mixture.

Bag for separation and isolation of blood components

Closed blood bag system for long term storage of red blood cells (RBCs) without contamination due to storage. System comprises the combination of a white blood cell (WBC) filter in sterile communication with a plastic bag made from a film substantially free of blood extractable plasticizers. In use, an RBC preservative solution (preferably from within the closed system) is added to a mixture of RBCs and WBCs and the mixture is then passed through the filter to remove at least 98% of the WBCs. The filtered RBCs and preservative solution pass into the attached bag and can then be stored in the attached bag for up to 42 days.

Red blood cell storage system

Closed filtering system for relatively fast and efficient removal of white blood cells from a red blood cell mixture. System comprises at least two blood bags in closed communication with each other via an intermediate filter assembly comprising a housing containing continuous filtering fiber. A preferred housing is tapered and the fiber preferably has a generally Y-shaped cross sectional area and it is adapted to permit substantial removal of white blood cells from a red blood cell mixture with minimal red blood cell hemolysis when the mixture is diluted with preservative solution and passed from one bag to the other at a relatively high flow rate. Filtration is completed within 24 hours, preferably within 6 hours, of whole blood donation.

Red blood cell filtering system

Closed filtering system for relatively fast and efficient removal of white blood cells from a red blood cell mixture. System comprises at least two blood bags in closed communication with each other via an intermediate filter assembly comprising a housing containing continuous filtering fiber. A preferred housing is tapered and the fiber preferably has a generally Y-shaped cross sectional area and it is adapted to permit substantial removal of white blood cells from a red blood cell mixture with minimal red blood cell hemolysis when the mixture is diluted with perservative solution and passed from one bag to the other at a relatively high flow rate. Filtration is completed within 24 hours, preferably within 6 hours, of whole blood donation.

Aqueous storage solution for red blood cells comprising a stabilized ascorbate derivative and having a pH sufficient to maintain substantially all of the initial concentration of ascorbate derivative and avoid formation of ascorbate degradation products during heat sterilization. A preferred solution includes at least about 90% of the pre-sterilization ascorbate and adenine but is free of dextrose.

Growing community of inventors

Concord, CA, United States of America

Raleigh A Carmen