Usv Private Limited

Rakesh Shekhawat

Divya Lal Saksena

Dhananjay Govind Sathe

Chandrakesan Muralidharan

Nikhil Umesh Mohe

Digamber Shripati Pawar

Avinash Venkatraman Naidu

Shrikant Mishra

Nilesh Dagdu Patil

Dipanwita Maiti

Subramanian Sundaram

Anjali Chutke

Priti Thakur

Niren Praful Thakar

Bipinchandra Rathod

Gulnaz Zaidi

Meera Sankarankutty

Uma Sankararaman

Sagar Satyanarayan Zawar

Dinesh Kumar Paliwal

Sanjay Vyankatrao Patil

Sukumar Ramanujam

Anindya Sibnath Bhattacharyya

Narasimha Kumar Kopparapu

Aruna Khare

Pankaj Prabhakar Malpure

Dilip Kumar Rana

Radharkrishnan Venkatsubramanian Tarur

Ramanujam Sukumar

Sundaram Subramanian

The present invention relates to analytical methods such as molecular weight determination of polypeptide, in particular Glatiramer acetate. The present invention further relates to an improved process for preparation of polypeptides or pharmaceutically acceptable salts thereof, particularly Glatiramer acetate also known as Copolymer-1. The present invention further relates to characterization of Glatiramer acetate by peptide mapping.

Copolymer-1, process for preparation and analytical methods thereof

The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol having the formula CHOwhere n is from 3 to 6, or a carbohydrate, or a derivative thereof wherein the concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1% to 10% w/w.

Process for gram scale production of PEG-r-metHuG-CSF

The present invention discloses a process for the preparation of rhPTH (1-34) also known as teriparatide by construction of a novel nucleotide, as an NcoI.IXhoI fragment as set forth in SEQ. ID. No.:1 encoding a chimeric fusion protein as set forth in SEQ.ID. No.:2 comprising of a fusion partner consisting of 41 amino acids belonging to&#x3b2;-galactosidase (LacZ) gene, an endopeptidase cleavage site, rhPTH (1-34) gene fragment, cloning the said nucleotide in an expression vector under the control of T7 promoter, transformingwith the said vector and expressing the chimeric fusion protein in fed batch fermentation. The present invention further discloses a low feed rate lactose induction for optimized expression of rhPTH (1-34) in. The present invention also discloses an unique, novel two step orthogonal purification process for rhPTH (1-34) comprising of cation exchange chromatography optionally followed by preparative chromatography selected from HIC or RP-HPLC to yield a target protein of &#x2267;99% purity. The present invention discloses a simple, cost-effective, environmentally benign method of producing high purity rhPTH (1-34).

Orthogonal process for purification of recombinant human parathyroid hormone (rhPTH) (1-34)

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Mumbai, India

Rakesh Shekhawat