National Research Council of Canada

Other

Raju S Datla

Gopalan Selvaraj

Jitao Zou

Wilfred A Keller

Yangdou Wei

William L Crosby

Ramesh B Nair

Joe K Hammerlindl

Prakash Venglat

Vivijan Babic

Maozhi Ren

Jun Huang

Wilf Keller

Richard W Joy, Iv

Tim Dumonceaux

Shuqing Qiu

Cyril Periappuram

Broad experimental tools that include biochemical molecular developmental global genomics and loss and gain of function transgenic approaches have been applied to address target of rapamycin (TOR) signaling pathway in plants especially usingmodel system andcrop Towards this objective, putative TOR interacting proteins (TIPs) have been identified and functions of these implicated in diverse developmental and biochemical processes have been investigated Functional studies including over-expression and silencing of TIPs have shown a range of phenotypes that include nutrition-use-efficiency, altered plant architecture and stress resistance in transgenicandlines Some of these phenotypes are relevant to important developmental pathways implicated in canola crop yield and performance.

Tor-interacting proteins (TIPs) and genes therefor

Late embryogenesis abundant (Lea) proteins accumulate in maturing seeds after many of the storage compounds have been synthesized, and they are considered relevant to maturation. We report here the molecular organization and expression of BnLea3-1, a novel Group 3 Lea gene from. BnLea3-1 contains a coding region of 798 bp, sharing 84.4% homology at the amino acid level with Lea76 of. Two tandem 11-mer repeats are truncated from the coding region of BnLea3-1, compared to the 13 conserved 11-mer repeats of Lea76. Substitutions of consensus residues are found at various positions within the 11-mer repeats. A 1561 bp 5' flanking promoter fragment of BnLea3-1 fused to&#x3b2;-glucuronidase (GUS) coding region conferred seed-specific GUS expression in stable transgenics of, tobacco and in transiently-transformed pea. A &#x2212;137 bp minimal promoter preceding the first transcription start site, identified through progressive deletions from the upstream was sufficient for basal GUS expression in the seeds and in leaves treated with ABA. Deletion studies indicate the presence of enhancing elements located between &#x2212;137 bp to &#x2212;742 bp and suppressing elements located between &#x2212;742 and &#x2212;1561 bp. BnLea3-1 expression in seeds precedes that of Lea76. Unlike other Group 3 Lea members including HVA1 and Dc3, BnLea3-1 is active in seeds and responsive weakly in vegetative tissues to ABA and methyl jasmonate (MeJA) but not to stress treatments. Possible functions of BnLea3-1 and another member of the Group 3 Lea family BnLea3-2 in embryo development is discussed.

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The present invention relates to methods for the use of the'BREVIPEDICELLUS' (BP) gene for alteration of plant architecture, in particular alteration of the morphology of the inflorescence of a flowering plant. The methods of the present invention provide a means to alter the development of the peduncle, notably the inflorescence branches, and the pedicels that subtend the individual flowers as well as aspects of flower structure such as the style, and subsequent seed pods, of a flowering plant. The invention also relates to methods to identify and isolate polynucleotides encoding genes with BP-related functions from other plant species and methods for utilizing said polynucleotides to alter the inflorescence of said plant species. Furthermore, the invention encompasses transgenic plants generated by the methods disclosed, and nucleotide sequences for use in generating the transgenic plants.

Methods for modification of plant inflorescence architecture

The invention provides a method for genetically transforming a plant so that it expresses a glycerol-3-phosphate dehydrogenase that is feedback-defective. The feedback-defective enzyme raises levels of glycerol and glycerol-3-phosphate in comparison to the wild-type, leading to increased osmotic stress tolerance, and altered fatty acid content in glycerolipids.

Transgenic manipulation of sn-glycerol-3-phosphate and glycerol production with a feedback defective glycerol-3-phosphate dehydrogenese gene

The sinapine content of seeds of , and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H:) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.

Transgenic reduction of sinapine in crucifera

Bifunctional genetic markers

Growing community of inventors

Saskatoon, Canada

Raju S Datla