International Genetic Engineering

Zoma Corporation

Jar-how Lee

Lindley C. Blair

Raju K. Koduri

Joachim L. Weickmann

Raju K Koduri

Jar-How Lee

Lindley Blair

Arnold H Horwitz

Joachim L Weickmann

Lindley C Blair

Genes are disclosed which are capable of directing the synthesis in a selected host microorganism of thaumatin I polypeptides having improved taste characteristics characterized by having the amino acid sequence of natural thaumatin I including a lysine amino acid residue substituted for asparagine in the 46th position from the amino terminal end, an aspartate amino acid residue substituted for asparagine in the 113th position from the amino terminal end and an aspartate amino acid residue substituted for lysine at either the 97th or 137th residue from the amino terminal end.

DNA encoding (Lys.sup.46, Asp.sup.97, Asp.sup.113) and (Lys.sup.46, Asp.sup

A shortened PGK promoter ranging from 165 to 404 base pairs of the Saccharomyces PGK promoter is taught. The promoter is operably linked to heterologous genes and improves the expression thereof relative to the full length PGK promoter.

Shortened phosphoglycerate kinase promoter

Growing community of inventors

Los Angeles, CA, United States of America

Raju K Koduri