US Government As Represented by the Secretary of the Army

Rajiv Pande

Jayant Kumar

David L Kaplan

Sukant K Tripathy

Joseph A Akkara

Kenneth A Marx

Madhu S Ayyagari

Sanjay Kamtekar

A method of detecting the presence of a substance being monitored in a medium, selected from the group of substances including organophosphorus compounds and the metal ions Zn, Be and Bi, including the steps of: providing a 1,2-dioxetane phenyl phosphate compound; providing a phosphatase that catalytically degrades the 1,2-dioxetane phenyl phosphate compound to produce light, the catalytic activity of the phosphatase toward 1,2-dioxetane phenyl phosphate compound being altered by the substance being monitored; exposing the 1,2-dioxetane phenyl phosphate compound and the phosphatase together to a medium which may contain the substance being monitored; detecting light produced after the exposing step; and determining, from the detected light, the presence and concentration in the medium of the substance being monitored.

Chemiluminescence-based method for rapid and sensitive in-situ detection

Growing community of inventors

Lowell, MA, United States of America

Rajiv Pande