10x Genomics, Inc.

Rajiv Bharadwaj

Anthony Makarewicz

Kevin D Ness

Serge Saxonov

Michael Schnall-Levin

Christopher Hindson

Bill Kengli Lin

Benjamin J Hindson

Tobias Daniel Wheeler

Augusto Manuel Tentori

Donald Masquelier

Mohammad Rahimi Lenji

Andrew D Price

Xinying Zheng

Hanyoup Kim

Katherine Pfeiffer

Debkishore Mitra

Yifeng Yin

Phillip Belgrader

Felice Alessio Bava

Pranav Patel

Paul Hardenbol

Zachary Bent

Steven W Short

Zahra Kamila Belhocine

Mirna Jarosz

Vijay Kumar Sreenivasa Gopalan

Alireza Salmanzadeh

Siyuan Xing

John R Stuelpnagel

Michael Ybarra Lucero

Tarjei Sigurd Mikkelsen

Geoffrey McDermott

Paul Ryvkin

Jessica Michele Terry

Josephine Harada

Cedric Uytingco

Elliott Meer

Niranjan Srinivas

David Maurice Cox

Indira Wu

Solongo B Ziraldo

Stephane Claude Boutet

Sarah Taylor

Christopher Joachim O'Keeffe

Zeljko Dzakula

Joakim Lundeberg

Denis Pristinski

Kamila Belhocine

Eswar Prasad Ramachandran Iyer

Jennifer Chew

Pratomo Alimsijah

Linda Kvastad

Martin Sauzade

Yiran Zhang

Ivan Akhremichev

Lynna Chen

Erica Graziosa

Nabil Mikhaiel

Mima Jarosz

Meghan L F Frey

Fernandino Valdecanas

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Methods of capturing multiple analytes on a spatial array

The present disclosure provides methods and systems for nucleic acid processing. A method for preparing a sequencing set may include providing a template nucleic acid and amplifying the template nucleic acid to provide a complementary nucleic acid. Next, the complementary nucleic acid may be fragmented and barcoded to produce a first set of barcoded fragments comprising a plurality of first barcoded fragments. Next, the plurality of first barcoded fragments may be fragmented to yield a second set of barcoded fragments comprising a plurality of second barcoded fragments.

Systems and methods for nucleic acid preparation

Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

Methods for sorting particles

Provided herein are materials and methods for in situ labeling of a biological sample for use in spatial analysis of one or more analytes in the biological sample. In some aspects, in situ labeling of a biological sample includes labeling the biological sample with one or more spatial barcodes.

In situ spatial barcoding

Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Devices, systems, and methods for increasing droplet formation efficiency

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

A method for aligning a sample to an array is provided. An image of sample image of a sample can be received by a data processor. The sample image having a first resolution. An array image including an overlay of an array with the sample and an array fiducial can be received by the data processor. The array image having a second resolution lower than the first resolution of the sample image. The sample image can be registered to the array image by aligning the sample image and the array image. An aligned image can be generated based on the registering. The aligned image can include an overlay of the sample image with the array. The aligned image can be provided by the data processor. A method for detecting fiducials associated with an array is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

Sample handling apparatus and image registration methods

The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

Electrophoretic methods for spatial analysis

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Droplet forming devices and system with differential surface properties

Devices and systems incorporating acoustic ordering and methods of use thereof

Imaging system hardware

Microfluidic channels networks and systems are provided. One network includes a first fluid channel having a first depth dimension; at least a second channel intersecting the first channel at a first intersection; at least a third channel in fluid communication with the first intersection, at least one of the first intersection and the third channel having a depth dimension that is greater than the first depth dimension. Also provided is a flow control system for directing fluids in the network. Systems are additionally provided for flowing disrupted particles into a droplet formation junction, whereby a portion of the disrupted particles or the contents thereof are encapsulated into one or more droplets. Further provided is a method for controlling filling of a microfluidic network by controlling passive valving microfluidic channel network features.

Microfluidic systems and methods of use

Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

Fluid delivery methods

Growing community of inventors

Pleasanton, CA, United States of America

Rajiv Bharadwaj