Oxford Glycosciences (UK) Ltd

Rajesh Bhikhu Parekh

James Alexander Bruce

Albert Edward Platt

Sally Barbara Prime

Robert Amess

Richard Michael Stoney

Dale Robert Pfost

David L Friedman

Lida H Kimmel

Robin Philp

Paul Nicholas Goulding

A method for analyzing a sample containing a plurality of analytes, comprises labelling the analytes with a detectable label, the label being chosen such that its detectability can be influenced by modifying the conditions of detection; separating the labelled analytes, before or after labeling,; and determining the presence of the separated analytes under the modified conditions and also, if desired, the unmodified conditions. Suitable detection apparatus comprises a support for a gel, means for detecting one or more analytes in the gel, and means for reducing the temperature of the gel to below ambient temperature, e.g., to no more than 0&deg;C.

Assaying and storing labelled analytes

The present invention provides computer-assisted methods and apparatus for identifying, selecting and characterizing biomolecules in a biological sample. A two-dimensional array is generated by separating biomolecules present in a complex mixture. A computer-readable profile is constructed representing the identity and relative abundance of a plurality of biomolecules detected by imaging the two dimensional array. Computer-mediated comparison of profiles from multiple samples permits automated identification of subsets of biomolecules that satisfy pre-ordained criteria. Identified biomolecules can be automatically isolated from the two dimensional array by a robotic device in accordance with computer-generated instructions. A supported gel suitable for electrophoresis is provided that is bonded to a solid support such that the gel has two-dimensional spatial stability and the solid support is substantially non-interfering with respect to detection of a label, such as a fluorescent label, associated with one or more biomolecules in the gel.

Robotic device for removing selected portions of a polyacrylamide gel

The present invention is directed to efficient, computer-assisted methods and apparatus for identifying, selecting and characterizing biomolecules in a biological sample. According to the invention, a biological sample is first treated to isolate biomolecules of interest, and a two-dimensional array is then generated by separating the biomolecules present in a complex mixture. The invention provides a computer-generated digital profile representing the identity and relative abundance of a plurality of biomolecules detected in the two-dimensional array, thereby permitting computer-mediated comparison of profiles from multiple biological samples. This automatable technology for screening biological samples and comparing their profiles permits rapid and efficient identification of individual biomolecules whose presence, absence or altered expression is associated with a disease or condition of interest.

Methods for computer-assisted isolation of proteins

Computer-assisted isolation and characterization of proteins

Computer-assisted methods and apparatus for identification and

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Near Wendlebury, United Kingdom

Rajesh Bhikhu Parekh