Oxford Glycosystems Limited

Rajesh B Parekh

James Bruce

Anthony H Merry

Sally B Prime

Stephen Alexander Charles

Albert Edward Platt

Antonis C Ioannides

Paul Goulding

David Harry Hawke

O-glycan is released from a glycoconjugate with a substantially anhydrous hydrazine reagent, the glycoconjugate being substantially salt-free and substantially anhydrous. Released O-glycan is recovered in substantially unreduced and intact form using a chromatographic material.

Release and isolation of O-glycans

Labelled carbohydrates and their use in assays

Sequencing of oligosaccharides

N- and O-glycans are released from a glycoconjugate with a substantially anhydrous hydrazine reagent, the glycoconjugate being substantially salt-free and substantially anhydrous. Released N- and O-glycans are recovered in substantially unreduced and intact form using a chromatographic material.

Release and isolation of N-glycans and O-glycans

An instrument that is particularly designed for the automatic release and isolation saccharides from glycoconjugates comprises a source of inert gas that can be passed along a line, to bring together all the required reagents with the sample, heat the sample in the presence of the reagents for the required time and at the required temperature, pass the resultant mixtures through chromatographic columns, and deliver the separated material to a collection point. The release and separation can be conducted on two samples of glycoconjugate simultaneously, even when the samples are of different glycoconjugates requiring different processing parameters. The material of one sample can be prevented from mixing with or otherwise contaminating the material of the other sample.

Automated process equipment

Growing community of inventors

Oxford, United Kingdom

Rajesh B Parekh