Council of Scientific and Industrial Research

Rajender Singh Sangwan

Girish Chandra Uniyal

Bali Ram Tyagi

Avdhesh Kumar Srivastava

Sushil Kumar

Ghulam Nabi Qazi

Rakesh Tuli

Krishan Avtar Suri

Dharam Chand Jain

Anil Kumar Dwivedi

Neelam Singh Sangwan

Ajay Kumar Mathur

Archana Mathur

Ritu Trivedi

Prabhat Ranjan Mishra

Avinash Kumar

Divya Singh

Srikanta Kumar Rath

Jyoti Gautam

Dharmendra Choudhary

Sulekha Adhikary

Usha Yadav

Vikram Khedgikar

Anita Gangwar

Mahesh Pal

Jiaur Rahaman Gayen

Anirudha Karvande

Payare Lal

Laxmi Narayan Misra

Narayan Das Chaurasiya

Priyanka Kushwaha

Rakesh Shukla

Prabodh K Trivedi

Shweta Sharma

Ashwni Verma

Sharad Sharma

Sarika

Kiran Khandelwal

Smrati Bhaduaria

Suresh Pratap Singh Gaur

Vivek Vidyadhar Bhosale

Hafsa Ahmad

A method for prevention and/or treatment of diminution of bone tissue using a composition comprising a compound selected from Withaferin A (WFA), Withanolide A, and Withanone. The composition showed enhanced WFA bioavailability in rodents against plain WFA, promotes bone marrow cell differentiation, and increases the percent of bone volume to tissue volume (BV/TV %) by 3 folds as compared to free Withaferin A (WFA).

Pharmaceutical composition for the treatment of diminution of bone tissue

The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from(Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.) (preferably 60% alcohol and 40% water) for the dry plant material and from 60% to 80% alcohol in the alcohol-water mixture for fresh plant material, the invented extractant composition leading to better/improved yields and accurate quantitative estimations of withaferin A existent in planta or in materia, selecting effective state of the tissue particularly use of the fresh materials improving yields, providing logistic benefits of isolations and diagnosis, and according several other advantages including technological, economic, preventing contaminations etc., the said method being valuable and advantageous for standardization of botanicals, metabolomic characterization of plants particularly chemotypes, analyzing metabolic transitions under physiological, genetical, environmental and biotic perturbations.

Process for isolation of withaferin-A from plant materials and products therefrom

The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.

Protein profiling of hyper acidic plants and high protein extraction compositions thereof

The invention provides a process for the induction of normal rooting on nodal and internodal stem segments without using hormones and/or other chemical treatments in Mentha species, which comprises oblique excision of shoots of Mentha species from the plants, immediately dipping the cut end of twigs or stems into water and keeping in complete dark for up to one week with by varying the temperature cycle for each set of 24 hours duration of the treatment.

Process for the induction of normal roots on nodes and internodes of stem segments without using hormone and/or chemical treatments in mentha species

The present invention provides a novel ecological method of phyto-remediation of alkaline ad chemically degraded soils, said method comprising the steps of dispersing biomass obtained from Pelargonium sp. in alkaline or chemically degraded soil.

Ecological method of phyto-remediation of alkaline and chemically degraded soils using scented geranium (Pelargonium sp.)

The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5&prime; CTG ATG CAT C3&prime;, 3&prime;,5&prime; TGG TCA CTG A 3&prime;, 5&prime; AGG GGT CTT G 3&prime;,5&prime; GAA ACG GGT G 3&prime;,5&prime; AGG GGT CTT G 3&prime;,5&prime; GCG TAA CGC C 3&prime;,5&prime; CAG CAC CCA C 3&prime;,5&prime; GTT GCG ATC C 3&prime;,5&prime; CAG GCC CTT C 3&prime;,5&prime; CGC AGT ACT C 3&prime;,5&prime; GTC CTA CTC G 3&prime;,5&prime; CTA CAC AGG C 3&prime; and 5&prime; GTC CTT AGC G 3&prime;, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.

Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin

The invention provides stable high ginsenoside-yielding callus lines of,(American Ginseng). The callus lines are useful in the industrial production of ginsenosides for use in a variety of ginseng preparations.

Stable high ginsenoside-yielding callus line of Panax quinquefolium (American ginseng) and a method for developing such stable high ginsenoside-yielding callus line

Growing community of inventors

Lucknow, India

Rajender Singh Sangwan