Sarnoff Corporation

David Sarnoff Research Center, Inc.

Powerzyme, Inc.

Rajan Kumar

Zvi Gerald Loewy

Zhonghui Hugh Fan

Peter John Zanzucchi

Satyam Choudary Cherukuri

Sterling Eduard McBride

Attiganal Narayanaswamy Sreeram

Richard Morgan Moroney, Iii

Michael James Liberatore

Leszek Hozer

Paul Heaney

Bryan Lloyd Bentz

Judith Ann Ladd

Chetna Bindra

Daniel Matt Fishman

Provided is a fuel cell comprising a first compartment, a second compartment and a barrier separating the first and second compartments, wherein the barrier comprises a proton transporting moiety.

Enzymatic fuel cell

Apparatus for separating molecules

The present invention relates to the denaturation of nucleic acids using capacitive charging. In a preferred embodiment, the invention effects such denaturation in the context of a microfluidic device for the analysis, amplification, or other automated manipulation of nucleic acids.

Capacitive denaturation of nucleic acid

The present invention relates to a method for capturing a class of nucleic acids comprising (a) providing a population of nucleic acids, wherein the population comprises the class; (b) binding a probe moiety to nucleic acids of the class, thereby forming one or more complexes, wherein the probe moiety is attached to a substrate; and (c) capturing the complex. The method can be implemented in certain embodiments in a structure comprised of one or more reservoirs, preferably wherein the reservoirs include one or more second chambers that are in communication with a first chamber. Preferably, the present invention is used to capture the class of nucleic acids that comprise regulatory elements included in a population of nucleic acids isolated from a cell, tissue, or organism. An alternative preferred embodiment of the present invention involves the capture of the class of nucleic acids that comprises certain repetitive elements included in a population of nucleic acids derived from an organism.

Method for capturing a nucleic acid

The nucleic acid amplification reactions of the present invention are conducted isothermally, using non-thermal denaturation procedures, such as occurs in chemical- or electrostatic-based denaturation. Embodiments are set forth for automated forms of the claimed procedures.

Microfluidic method for nucleic acid amplification

The nucleic acid amplification procedures of the present invention provide methods for amplification of a nucleic acid comprised of a first strand, comprising (a) using the first strand to generate copies of a second strand at a first location, (b) moving the copies of the second strand to a second location, and (c) using the copies of the second strand to generate copies of at least a portion of the first strand. Target nucleic acids used in the context of the present method include RNA or DNA, either single stranded or double stranded, using primer extension or joining-type protocols. Embodiments are set forth for automated forms of the claimed procedures.

Amplification method for a polynucleotide

The present invention relates to the detection of a polynucleotide using a padlock probe, whereby a hybridization process is combined with ligation to irreversibly capture polynucleotides of interest. The invention relates both to the padlock probe itself, as well as to methods of use, which allow for varying levels of sensitivity due to amplification of signal processes built into the system. Additionally, preferred formats are disclosed for use of the padlock probes, including the use of microfluidics.

Padlock probe detection

In one aspect, described are methods of sequencing of polynucleotides using a sequential step procedure. The methods of the invention begin with the provision of a single-stranded polynucleotide template that is annealed with a primer, forming a template-primer complex. The methods of the invention can provide for adding discrete nucleotides by a polymerase to the template-primer complex, where the added nucleotides can be labeled. The methods of the invention can involve the identification of a polynucleotide or polynucleotides having a contiguous non-redundant string or a superimposed non-redundant string pattern. The detection of the presence of a non-redundant contiguous string can be used, for example, to identify a particular gene. Alternatively, for example, if the non-redundant contiguous string is not unique to a particular gene, the string can be used to form a DNA library that can then be searched, for example, with a second string. Similarly, a superimposed non-redundant string pattern can be used, for example, to identify a gene or to search a DNA library. Such strings can be used, for example, in annealing reactions and in computer searches of a database having a catalog of sequenced polynucleotides.

Sequential step method for sequencing and identifying polynucleotides

The invention provides nuclease protection assay comprising: (A) attaching a nucleic acid probe comprising a first nucleotide sequence to a solid surface area; (B) contacting the nucleic acid probe with a nucleic acid template under conditions that promote hybridization between complementary polynucleotides, forming a probe-template complex if the template includes a segment that is complementary to the probe; (C) contacting the probe-template complex with a nuclease effective to selectively cleave the nucleotide bonds of (1) the first nucleotide sequence when the first nucleotide sequence is single stranded or (2) mismatched regions of the first nucleotide sequence when the first nucleotide sequence is in duplex nucleic acid; and (D) detecting the presence of duplex nucleic acids formed by the probe and template nucleic acids by detecting the presence of the first nucleotide sequence.

Growing community of inventors

Robbinsville, NJ, United States of America

Rajan Kumar