Universiti Putra Malaysia

Raja Noor Zaliha Raja Abd Rahman

Abu Bakar Salleh

Mahiran Basri

Randa Abdelkareem Abusham

Adam Leow Thean Chor

A method for crystallizing astrain T1 polypeptide under gravity or microgravity conditions is provided. A purified protein to be crystallized is provided, the purified protein being either thermostable T1 lipase or thermostable F16L lipase. A protein solution is prepared, comprising the purified protein, a buffer solution having a working pH of at least 8.5, and between 3-5% glycerol. A reservoir solution is prepared, comprising 0.1M MES, 0.1M NaH2PO4, 0.1M KH2PO4, and 1 M NaCl, and having a working pH of 6.5 or 6.6. The protein and reservoir solutions are combined to obtain a protein droplet, and protein crystals are obtained from the protein droplet by hanging-drop vapor diffusion.

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The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating fromisolate Rand bacteria. The activity and stability of protease (preferably named Rand protease) at high temperature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique.

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Selangor, Malaysia

Raja Noor Zaliha Raja Abd Rahman