Universiti Putra Malaysia

Universiti Purta Malaysia

Raja Noor Zaliha Abd Rahman

Abu Bakar Salleh

Mahiran Basri

Mohd Basyaruddin Abd Rahman

Halila Jasmani

Mohd Zobir Hussein

Siti Salhah Othman

Leow Thean Chor

Jalimah Binti Yunus

Immobilization oflipase on a carrier selected from the group consisting of macroporous adsorbent resin of the acrylic type, synthetic epoxy activated resin and Mg&#x2014;Al-hydrotalcite enhances its enantioselectivity by six to seven folds. The immobilizedlipase is suitable for use in resolution of racemic alcohols and/or carboxylic acids, particularly in resolution of racemic menthol or production of menthyl esters.

Method of producing enantioselective immobilized candida rugosa lipase

Wax esters are prepared by reacting a solution of palm oil in an organic solvent with oleyl alcohol in the presence of an immobilized lipase wherein molar ratio of palm oil to oleyl alcohol is between 1:2 and 1:4. Immobilized lipase used is present in an amount which is equivalent to not less than 1000 &#x3bc;g of protein per milli-mole of palm oil used and the reaction is carried out at 40&#xb0; C. to 50&#xb0; C. for a period of not less than 5 hours. The organic solvent used has a value of log P not less than 3.5.

Production of wax esters

Enantioselective immobilized lipase

A biologically pure culture ofStrain T1 bacteria isolated from Palm Oil Mill Effluent capable of producing thermostable lipase T1 gene. The production of thermostable lipase T1 gene from a novelbacterium having a designated as strain T1, was aerobic, gram positive and endospore-forming, rod-shaped. The G+C content of the genomic DNA was 52.6%. On the basic of physiological data, phenotypic traits and molecular analysis, strain T1 represents a novel species within the genus, The thermostable T1 lipase gene was subcloned into the pGEX-4T1 vector with and without signal peptide in prokaryotic system. The 28 amino acid residues signal peptide alter the conformation of GST moiety and preventing it from bind to affinity glutathione Sepharose column. By expression and simplification of the purification through single step affinity chromatography shows a specific activity of 292.929 U/mg and 72.55% of fusion lipase was recovered from crude cell lysate. T1 mature lipase was successfully purified with a final recovery of 51.49% and specificity activity of 959.033 U/mg.

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Growing community of inventors

Selangor Darul Ehsan, Malaysia

Raja Noor Zaliha Abd Rahman