Monsanto Company

Raj B Parekh

Raymond A Dwek

Thomas William Rademacher

Joseph Feder

William R Tolbert

Arthur J Wittwer

Susan C Howard

Mark R Wormald

Christopher J Edge

A method of oligosaccharide sequencing in which the components are determined essentially simultaneously is disclosed which comprises a series of steps as follows: A. Placing an identifying label on the reducing terminal residue of the oligosaccharide to be sequenced, B. Dividing said oligosaccharide into a plurality of separate portions of known integer amounts, C. Treating each said portion with a different reagent mix to thereby provide a series of reaction mixtures, D. Pooling known integer amounts of the products from each separate reaction mixture to give a product pool, E. Performing an analysis on said product pool which measures the molar proportions of the reaction products, and F. Reconstructing or identifying the starting oligosaccharide from the molar prevalence of said reaction products.

Oligosaccharide sequencing

Normal human colon fibroblast tissue plasminogen activator is separated by lysine-Sepharose affinity chromatography into Types I and II glycoforms and characterized with respect to the relative incidence of each type of oligosaccharide comprising the respective Types I and II glycoforms.

Tissue plasminogen activator from normal human colon cells

Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co).

Tissue plasminogen activator oligosaccharide from normal human colon

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Oxford, United Kingdom

Raj B Parekh