Bayer Aktiengesellschaft

Meiji Seika Kaisha, Ltd.

Institut Fuer Bioanalytik, Umwelt-toxikologie Und Biotechnologie Halle Gmbh

Rainer Zocher

Horst Kleinkauf

Peter Jeschke

Norbert Mencke

Achim Harder

Takeshi Murakami

Gerhard Bonse

Naomi Sumida

Katsuharu Iinuma

Winfried Etzel

Konrad Glund

Naoki Midoh

Koichi Miyamoto

Gerhard Thielking

Matthias Hoffmann

Wolfram Weckwerth

Kouichi Miyamoto

An objective of the present invention is to provide a D-phenyllactic acid dehydrogenase. Another objective of the present invention is to provide a gene which encodes the D-phenyllactic acid dehydrogenase. A D-phenyllactic acid dehydrogenase according to the present invention is a protein comprising an amino acid sequence of the amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that has one or more modifications selected from a substitution, a deletion, an addition and an insertion and has D-phenyllactic acid dehydrogenase activity. A D-phenyllactic acid dehydrogenase gene according to the present invention comprises a nucleotide sequence which encodes the D-phenyllactic acid dehydrogenase, for example the nucleotide sequence of SEQ ID NO: 1.

(R)-2-hydroxy-3-phenylpropionate (d-phenyllactate) dehydrogenase and gene encoding the same

A method for the continuous synthesis of baccatin-III is performed by means of the enzymatic acetylations of 10-deacetyl baccatin-III (10-DAB) in an enzyme reactor. This objective is accomplished by a method, for which an aqueous reaction batch is used, which contains a partially purified or a very pure acetyl transferase from a Taxus species and synthesis components, especially 10-DAB, acetyl compounds or optionally an acetyl coenzyme A, which can be regenerated, a protective colloid and optionally a buffer substance in solution. The solution is separated by a semipermeable membrane from an organic solvent, which serves as extraction agent for baccatin-III, the reaction batch being regenerated at regular intervals in an aqueous buffer system and the solvent continuously being exchanged. By conducting the reaction, the product can be removed continuously from the reaction batch for the partial synthesis of taxol and taxoter. By the above method, achievable yields are increased significantly.

Method for the production of baccatin-III by enzymatic synthesis

Process for the preparation of substituted aryl lactic acid containing

The present invention relates to a process for the preparation of lactic-acid-containing, optically active, cyclic depsipeptides having 18 ring atoms with the aid of fungal strains of the species Fusarium or enzymatic preparations isolated therefrom.

Lactic-acid-containing cyclic depsipeptides having 18 ring atoms as

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Berlin, Germany

Rainer Zocher