Eppendorf Ag

Rainer Schliesser

Andreas Schirr

Rainer Treptow

Gerd Joachim Eckert

Norbert Wittschief

A method for the quantitative real time analysis of fluorescent samples is provided, at which each of the samples is excited to fluoresce by a sample individual light source () and the intensity of the light which is emitted by the samples is measured. For a highly precise measurement of even low light intensities for the purpose of reduction of the analysis time, each light source () is switched on and off-during a defined interval by a clocked pulse sequence of constant pulse frequency alternately. The measurement of the intensity of the emission light during these intervals is exclusively performed during the switch-on phases of the light, source ().

Method and device for the quantitative real time analysis of fluorescent samples

The invention relates to a method for photometrically investigating sample radiations of at least one sample, which are caused by the radiation of N emitter elements of at least one radiation element wherein said N emitter elements are emitting radiation during time periods which at least partially overlap, to detect the sample radiation of at least two samples as a sum signal during time periods which at least partially overlap and to evaluate the sample radiation of at least one individual sample from said sum signal.

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Stuhr, Germany

Rainer Schliesser