Roche Diagnostics Gmbh

Rainer M&uuml;ller

Urban Schmitt

Richard Engh

Hans Brandstetter

Arno Deger

Erhard Kopetzki

Rainer Schmuck

Alfred Engel

Harald Weisser

Kerstin Kr&uuml;ger

Eberhard Kopetzki

The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.

Recombinant inactive core streptavidin mutants

Recombinant inactive avidin mutants

DNA molecule comprising a structural gene which codes for a bacterial protein with the enzymatic activity of 3-hydroxybutyrate dehydrogenase (3-HBDH), a recombinant DNA containing the DNA molecule, microorganisms transformed accordingly, a process for obtaining 3-HBDH by culturing a suitable transformed microorganism as well as a method for the determination of ketone bodies in the presence of the enzyme. A structural gene from Rhodobacter sphaeroides is preferred.

Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof

Growing community of inventors

Penzberg, Germany

Rainer Müller

Growing community of inventors

Penzberg, Germany

Rainer M&uuml;ller

Rainer Müller