Fei Comapny

Life Technologies Corporation

Fei Deutschland Gmbh

Till I.d. Gmbh

Thermo Fisher Scientific (bremen) Gmbh

Thermo Fisher (bremen) Gmbh

Rainer Daum

Rainer Uhl

Xaver Voegele

Alexander Alekseevich Makarov

Michael D Ward

Christian Seebacher

Rudolf Johannes Peter Gerardus Schampers

Johannes Antonius Hendricus Wilhelmus Gerardus Persoon

Gerrit Pahl

Bruce Pirger

Tobias Mayer

Matthias Geisbauer

Xaver Vögele

An imaging system having one or more single-photon detectors is configured to enable rapid switching between different operational modes. The imaging system includes a reprogrammable controller communicatively coupled to one or more single-photon detectors and to a host computer device via a high-speed data link. In operation, the one or more single-photon detectors are operated according to a first operational mode. A reprogrammable logic device of the controller is then reconfigured to operate the one or more single-photon detectors in a second operational mode without requiring changes to the overall hardware configuration/setup of the imaging system.

Single-photon imaging system with reconfigurable controller for rapid switching between imaging modes

A system for selectively adjusting an objective lens correction collar includes a gear ring sized and shaped to fit about a correction collar of an objective lens. The system also includes an adjustment mechanism that has a motor operably connected to a complementary gear configured to selectively engage and cause movement of the gear ring, thereby adjusting the correction collar.

Systems and methods for motorized adjustment of objective lens correction collar

Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device includes an optical assembly, a sample stage, and a receiver. The optical assembly enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume toward the receiver. A laser focus detection unit including at least one reference laser and photodetector generates at least one real-time detection signal indicative of one or more characteristics of the sample during ablation and/or of a distance from the objective to the sample stage or a surface of the sample. A controller coupled with the laser focus detection unit dynamically controls in real-time one or more parameters of the ablation laser and/or a position of the objective and/or a position of the receiver relative to the sample to improve MS imaging quality.

Systems and methods for imaging and ablating a sample

Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device () includes an optical assembly (), a sample stage (), and a receiver (). The optical assembly () is disposed in an inverted position below the sample stage () and the receiver () is positioned above the sample stage (). The optical assembly enables imaging of a sample disposed on the sample stage (). The optical assembly () also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume () toward the receiver ().

A standing wave interferometric microscope is disclosed herein. An example microscope may include an illuminator, for illuminating a specimen with a standing wave of input radiation at an analysis location to cause the specimen to fluoresce, the specimen arranged in the analysis location, a pair of projection systems, arranged at opposite sides of the analysis location, coupled to collect at least a portion of the fluorescence and direct a corresponding pair of fluorescence light beams into a respective pair of inputs of an optical combining element, a wavefront modifier for producing astigmatism in at least one of the fluorescence light beams entering the optical combining element, and a detector for examining output light from said combining element.

Standing wave interferometric microscope

A wide-field interferometric microscope comprising:

The invention relates to a microscope arrangement provided with: a microscope () having at least two optical outputs () for outputting a fluorescence signal and a switching arrangement () for switching the output of the fluorescence signal between the optical outputs; a beam splitter arrangement (); optical elements () for generating a separate partial beam path () associated with each output in such a way that the respective fluorescence signal of each of the outputs is superimposed at the beam splitter arrangement after passing through the respective partial beam path; and also at least two optical detectors (), wherein for each of the partial beam paths, one of the detectors is located behind the beam splitter, seen from the microscope, in reflection and another of the detectors is located behind the beam splitter arrangement, seen from the microscope, in transmission.

Switchable microscope arrangement with multiple detectors

The present invention relates to a method of studying a sample using an optical microscope, comprising providing the sample in a sample holder with means to maintain the sample at a temperature below 273 K; providing a microscope objective lens, in a thermally insulating jacket, having an extremal lens element proximal to the sample holder; bringing the lens into a focus position proximal to the sample, which separates the extremal lens element and sample by an intervening space, providing a transparent window in said intervening space, with a gap between the window and the extremal lens element; providing a flow of substantially dry gas in said gap; and tailoring the geometry and velocity of said flow so that, at least in said gap, the flow is non-laminar; and does not excite substantial acoustic vibration in a structure proximal the gap.

Method of studying a cryogenic sample in an optical microscope

A device for confocal observation of a specimen, having a mask, which is located in the illumination beam path and the image beam path and is rotatable around a central axis, the mask being provided with openings for generating an illumination pattern moving on the specimen, an arrangement of a plurality of focusing microoptics which is adjusted to the geometric arrangement of the openings of the mask and to the rotation of the mask in order to concentrate the illumination light by each of the microoptics into a respective one of the openings of the mask, and a beam splitter for separating light from the specimen from illumination light, wherein the beam splitter is arranged in the beam path between the mask and the arrangement of the microoptics, and wherein an optical arrangement is provided in the beam path between the mask and the arrangement of the microoptics.

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Wessling, Germany

Rainer Daum