Sophion Bioscience A/s

Rafael Taboryski

Jonatan Kutchinsky

Morten Bech

John Robert Dodgson

Nicholas Oswald

Bent E Nielsen

John Shaw

Lars Thomsen

Ras Kaas Vestergaard

Niels Willumsen

Dirk Reuter

Jørgen Due

Lars c/o Thomsen Bioscience Thomsen

Bent Erling Nielsen

J Rgen Due

A microfluidics system comprising a channel having an inlet () and an outlet (); a first membrane () positioned between the inlet () and outlet () and comprising an aperture having a radius within the range 0.1 to 50 &#x3bc;m, the inlet () and the outlet () being in hydraulic communication with one another, such that a fluid can move along the channel from the inlet to the outlet.

Capillary stop

The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane, The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.

Substrate and method for measuring the electro-physiological properties of cell membranes

The present invention provides a pump for generating an Electroosmotic Flow (EOF) in a solution in a canal, guide, pipe or equivalent. Electroosmotic flow is generated by application of an electric field through a solution in a canal defined by insulating walls. The phenomenon depends on ionisation of sites on the surface so that for electroneutrality there is an excess mobile charge in the solution. The electric field acts on the excess charge in the solution causing the fluid to flow. The quantity and distribution of excess charge in the solution depends on the solution and the surface materials and is related to a parameter, the zeta (z) potential characterising material/solution combinations.

Corbino disc electroosmotic flow pump

The present invention relates to a high through-put system for determining and/or monitoring electrophysiological properties of ion channels of ion channel-containing membranes, typically lipid membrane-containing structures such as cells. In particular, the invention provides a substrate which provides means for automatically positioning cells at measuring sites using electroosmotic flow in canals formed on or in the substrate. The electroosmotic flow is generated and controlled by electroosmotic flow pumps integrated on the substrate or positioned in relation thereto. Thereby, cells can be positioned in favorable measurement configuration at a plurality of sites for performing testing and measurements. Also, the invention relates to a main electric circuit for performing testing and measurements on a plurality of cells in parallel.

System for electrophysiological measurements

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Lyngby, Denmark

Rafael Taboryski