Empresa Brasileira De Pesquisa Agropecuaria-embrapa

Rafael Perseguini Del Sarto

Maria Cristina Mattar Da Silva

Thales Lima Rocha

Maria Fatima Grossi De Sa

The present invention relates to the field of insect pests control, using methods and compositions which comprise of alpha-amylase analogous mutant inhibitors (&#x3b1;AIs). More specifically, the invention provides new &#x3b1;AIs analogous mutant molecules for controlling insect pests, in particular boll weevils (), partially or totally presenting reduction of the amylolytic activity of the digestive enzymes in the intestinal lumen of the insect. Other aspects of the invention include gene constructs containing the nucleic acid molecules that code for the alpha-amylase inhibitors, heterologous expression methods of the new molecules in the active form, and the use of these molecules for controlling insect pests. The invention also provides analogous synthetic genes optimized for their transformation and expression in plants.

Alpha amylase mutant inhibitors isolated from phaseolus vulgaris with properties of controlling insect pests, compositions containing such mutants, and method of using thereof

Growing community of inventors

Brazilandia, Brazil

Rafael Perseguini Del Sarto