Ge Healthcare Bio-sciences Ab

Amersham Biosciences Ab

Ge Healthcare Bioprocess R&d Ab

Raf Lemmens

Klaus Gebauer

Ola Lind

Kajsa Stridsberg-Friden

Jamil Shanagar

Jan Berglof

Joachim Stadler

Elisabeth Hallgren

Bjorn Johan Lundgren

Tomas Nyhammar

Helena Widegren

Björn Lundgren

Bjoern Lundgren

Kajsa Stridsberg-FRIDÉN

The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

Method for dry packing chromatography columns

The present invention is a method of separating nucleic acid molecules from contaminants, such as proteins, in a solution and isolating one or more desired nucleic acid molecules, which method comprises the following steps (a) providing an aqueous adsorption solution, which includes nucleic acids and a salt that forms lyotropic ions when dissolved; (b) passing said solution over a matrix to adsorb the nucleic acids onto the matrix, said matrix including an aromatic ring moiety and at least one thioether moiety; (c) passing an aqueous eluent over said matrix to desorb the nucleic acid molecules therefrom, which eluent includes a salt that forms lyotropic ions and a gradient of increasing ionic strength originating from an increasing concentration of a salt that forms less lyotropic ions when dissolved than the ones present in said aqueous adsorption solution; and (d) isolating a fraction comprising the desired nucleic acid molecules.

Method of separation using aromatic thioether ligands

The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

Isolation of DNA molecules

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Uppsala, Sweden

Raf Lemmens