Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Rachel Shahinian

Thomas W Myers

Keith Bauer

Fred Lawrence Reichert

Shawn Lee Suko

Joseph San Filippo

Stephen Gordon Will

Ellen H Fiss

Veeraiah Bodepudi

Shawn Suko

Fred Reichert

Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

DNA polymerases with improved activity

Methods for conducting primer extension using DNA polymerases with improved activity

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

Alkyl amines improve detection of components of formaldehyde-fixed biological samples

Growing community of inventors

Alameda, CA, United States of America

Rachel Shahinian