Danisco US Inc.

The Goodyear & Tire Rubber Company

Rachel E Muir

Joseph Charles McAuliffe

Dmitrii V Vaviline

Caroline M Peres

Walter Weyler

Derek H Wells

Alex Toftgaard Nielsen

Gopal K Chotani

Michael C Miller

Jimmy Chan

William Throndset

The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.

Microtiter plates for controlled release of culture components to cell cultures

This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.

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Enhanced production of isoprene using host cells having decreased ispA activity

The invention provides, inter alia, compositions and methods for the biological production of pentose sugars, such as 2-methylerythritol (2-ME), 1-deoxyxylulose (1-DX), and monoacetylated-2-C-methylerythritols, using recombinant cells.

Biological production of pentose sugars using recombinant cells

The invention provides for compositions and methods for producing isoprene by using recombinantly engineered cells that utilize a system of dual IspG enzymes in addition to isoprene synthase.

Compositions and methods for improved isoprene production using two types of ISPG enzymes

The invention provides for methods for producing isoprene from cultured cells using various components of the DXP pathway and MVA pathway, or components associated with the DXP pathway and MVA pathway, iron-sulfur cluster-interacting redox polypeptides, and isoprene synthase. The invention also provides compositions that include these cultured cells.

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Redwood City, CA, United States of America

Rachel E Muir