Caribou Biosciences, Inc.

University of California

Rachel E Haurwitz

Jennifer A Doudna

Andrew Paul May

Paul Daniel Donohoue

Matthew Merrill Carter

James M Berger

Martin Jinek

Blake Wiedenheft

John Van Der Oost

Daniël Christianus Swarts

Lei S Qi

Peter Sean Cameron

Adam P Arkin

Megan Van Overbeek

Christopher H Nye

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof.

Compositions and methods of nucleic acid-targeting nucleic acids

This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof.

Methods of modifying a target nucleic acid with an Argonaute

Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.

Methods for increasing CAS9-mediated engineering efficiency

Methods of modifying a target nucleic acid with an argonaute

The present disclosure provides nucleic acids encoding an RNA recognition sequence positioned proximal to an insertion site for the insertion of a sequence of interest; and host cells genetically modified with the nucleic acids. The present disclosure also provides methods of modifying the activity of a target RNA, and kits and compositions for carrying out the methods.

Methods and compositions for controlling gene expression by RNA processing

The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.

Endoribonuclease compositions and methods of use thereof

Compostions and methods of nucleic acid-targeting nucleic acids

This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. Genome engineering can refer to altering the genome by deleting, inserting, mutating, or substituting specific nucleic acid sequences. The altering can be gene or location specific. Genome engineering can use nucleases to cut a nucleic acid thereby generating a site for the alteration. Engineering of non-genomic nucleic acid is also contemplated.

Growing community of inventors

Kensington, CA, United States of America

Rachel E Haurwitz