King Saud University

Rabab Abd El Moneim Khalil El Dib

Shaza Mohamed Adel Al-Massarani

Manal Ahmed Gasmelseed Awad

Ali Ali El-Gamal

Ali Ali Hasan Elgamal

Awatif Ahmed Hendi

Khalid Mustafa Osman Ortashi

Adnan Jathlan Al-Rehaily

Manal Ahmed Gasmelseed

Ali Ali Hasan El Gamal

The synthesis of ursolic acid nanoparticles includes dissolving ursolic acid powder in methanol, boiling water for five minutes, and adding the methanol solution to the boiled water dropwise at a flow rate of 0.1-0.3 ml/min under ultrasonic conditions. After sonication for 20 minutes, the contents are stirred for about 15 minutes, and then dried. Particle size distribution studies and TEM micrographs confirm the resulting product comprises nanoparticles. In vitro testing confirms the ursolic acid nanoparticles exhibit greater anticancer activity than conventional-size particles, and that the nanoparticles exhibit antimicrobial effect against gram positive and gram negative bacteria, as well as fungi.

Synthesis of ursolic acid nanoparticles

In one embodiment, synthesis ofnanoparticles includes providing apowder; dissolving the powder in a first alcohol to provide a first alcohol extract; concentrating a filtrate from the first alcohol extract under reduced pressure to provide a dried alcohol extract; dissolving the dried alcohol extract in the first alcohol to provide a second alcohol extract; successively partitioning the second alcohol extract using n-hexane to provide an n-hexane extract; dissolving the n-hexane extract in a second alcohol and water to provide a first solution; and adding an acidic solution to the first solution to form a final solution includingnanoparticles.

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A method for synthesizing 3-oxolupenal nanoparticles including isolating 3-oxolupenal from a fraction ofplant, reducing the 3-oxolupenal to obtain a powder of 3-oxolupenal, dissolving the powder of 3-oxolupenal in methanol to form a first solution, adding the first solution to boiling water to form a second solution, sonicating the second solution, and freeze-drying after sonication to obtain the synthesized 3-oxolupenal nanoparticles. The synthesized 3-oxolupenal nanoparticles exhibited cytotoxic effects and antimicrobial effects.

Method of synthesizing of 3-oxolupenal nanoparticles

Synthesis ofnanoparticles includes providing an extract of, dissolving the extract in alcohol to provide a mixture, adding water to the mixture to provide an aqueous solution, adding an acidic solution to the aqueous solution to form a final solution includingnanoparticles; and centrifuging the final solution to isolate thenanoparticles.

Synthesis of ifflaionic acid nanoparticles includes dissolving a powder of ifflaionic acid in an alcohol solution to form a first solution, adding the first solution to an aqueous solution under ultrasonic conditions to produce a sonicated solution, stirring the sonicated solution for a duration of time to produce a mixture, and freeze-drying the mixture to provide the ifflaionic acid nanoparticles.

Synthesis of ifflaionic acid nanoparticles

A method of preparing nanoparticles from desert date can include providing a metal salt solution comprising metal ions; providing desert date extract solution that comprises a reducing agent, and combining the metal ion solution and the desert date extract solution while stirring at a temperature in the range of 25&#xb0; C. to 100&#xb0; C. to produce metal or metal oxide nanoparticles. The metal nanoparticles can be gold nanoparticles. The metal oxide nanoparticles can be zinc oxide nanoparticles. The nanoparticles can be used to inhibit the growth or proliferation of a cancer cell and/or microorganisms.

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Giza, Egypt

Rabab Abd El Moneim Khalil El Dib