Amgen Inc.

Tularik Limited

R Marc Learned

Steven L McKnight

Bei Shan

Jin-Long Chen

M Catherine Amaral

Fabienne Charles De La Brousse

Fabienne C De La Brousse

The invention provides methods and compositions for screening for pharmacological agents which regulate gene expression in mammals. An exemplary assay involves (a) contacting a mammalian cell comprising a knock-in mutant of a targeted native allele encoding a reporter of gene expression, wherein the expression of the reporter is under the control of the gene expression regulatory sequences of the native allele, with a candidate agent under conditions whereby but for the presence of the agent, the reporter is expressed at a first expression level; and, (b) measuring the expression of the reporter to obtain a second expression level, wherein a difference between the first and second expression levels indicates that the candidate agent modulates gene expression.

Cell-based drug screens for regulators of gene expression

Growing community of inventors

El Granada, CA, United States of America

R Marc Learned