University of California

Lawrence Livermore National Security, LLC

National Institute of Infectious Disease

Tsukuba Primate Research Center, National Institute of Biomedical Innovation

R Holland Cheng

Li Xing

Chun Chieh Chen

Marie Stark

Mohammad Ali Baikoghli

Kit S Lam

Matthew A Coleman

Juntao Luo

Tatsuo Miyamura

Paul Henderson

Wei He

Naokazu Takeda

Yasuhiro Yasutomi

Tian-Cheng Li

Marie Christine Stark

A Hepatitis E virus (HEV)-based virus like nanoparticle (HEVNP) made with a modified capsid protein containing at least a portion of open reading frame 2 (ORF2) protein conjugated with gold nanocluster is provided. Also provided are methods of targeted delivery of a nucleic acid using the HEVNP.

Virus-like nanoparticles for oral delivery

Modified capsid proteins containing at least a portion of hepatitis E virus (HEV) open reading frame 2 (ORF2) having one or more cysteine residues in a surface variable loop or the C-terminus of HEV ORF2, or a portion thereof, are provided. The modified capsid proteins can be used to form hepatitis E virus (HEV) virus like particles (VLPs) having cysteine functional groups exposed on the outer-surface. The exposed cysteine functional groups can be modified via their thiol reactive group. For example, a bioactive agent, such as a cell-targeting ligand, can be conjugated to the one or more cysteines for targeted delivery of chemically activated nanocapsids.

Chemically activated nanocapsid functionalized for cancer targeting

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R), wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a poly(ethylene glycol) polymer; each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.

Apolipoprotein nanodiscs with telodendrimer

This invention provides a peptide/nucleic acid composition for oral/mucosal, dual-modal activation of immune protection systems.

Proteolysis-resistant capsid of chimeric hepatitis E virus as an oral delivery vector

Multiple antigen delivery system using hepatitis E virus-like particle

Growing community of inventors

Davis, CA, United States of America

R Holland Cheng