Shandong University

Shandong Institute for Food and Drug Control

Avanc Pharmaceutical Co., Ltd

Qunye Zhang

Feng Shi

Lianli Chi

Baojian Hang

Ruiqing Xian

Weijian Wang

Liping Gong

Congcong Wang

Fengshan Wang

Hongming Du

The present invention provides a snake venom thrombin marker peptide ofand an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme ofand improves the quality control level.

Snake venom thrombin-like enzyme marker peptide ofand its application in the species identification of hemocoagulase for injection

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Shandong, China

Qunye Zhang