Bio-rad Laboratories, Inc.

Raindance Technologies, Inc.

Other

Qun Zhong

Darren Roy Link

Jonathan William Larson

Benjamin J Miller

Mark Weary

Andrew Wilson

Yevgeny Yurkovetsky

Brian Hutchison

Andrew Watson

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Digital analyte analysis

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

Manipulation of microfluidic droplets

The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

Manipulating droplet size

Analyzing microdroplet outline size and adjusting channel pressure to alter microdroplet size

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Lexington, MA, United States of America

Qun Zhong