Jiangsu Academy of Agricultural Sciences

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Snu Rdb Foundation

Qiyan Xiong

Zhixin Feng

Li Wang

Yun Bai

Yuzi Wu

Guoqing Shao

Yanfei Yu

Maojun Liu

Beibei Liu

Yanna Wei

Yuan Gan

Lei Zhang

Zhanwei Wang

Daesub Song

Rong Chen

Jongwoo Lim

Jia Wang

Lizhong Hua

Haiyan Wang

Long Zhao

Fangfang Bai

Fei Hao

Minjoo Yeom

Lulu Xu

Wenliang Li

Yongjie Liu

Daohua Zhang

Xing Xie

Guoqinq Shao

Dongxia Liu

A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 10TCID/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.

Viral vector delivery system for both respiratory and digestive tracts of pigs and application thereof

A composition and a kit for detectingare provided. The composition for detectingis an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5' terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of

Composition and kit for detecting mycoplasma

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical(Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.

Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

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Nanjing, China

Qiyan Xiong