Syngenta Participations Ag

Syngenta Crop Protection Ag

Qiudeng Que

Moez Rajabali Meghji

Jeng Shong Chen

Zhongying Chen

Mary-Dell Matchett Chilton

Heng Zhong

Weining Gu

Timothy Kelliher

Hope Prim Hart

Derrick Pulliam

Nykoll Long

Ailing Zhou

Myoung Kim

Yaping Jiang

Mark Scott Rose

Peter Bernard Heifetz

Joseph Dallas Clarke, V

Annick Jeanne De Framond

Jeffrey Bottoms

Yinping Lucy Qin

Johannes Jacobus Ludgerus Gielen

Petrus Theodorus De Haan

David Andrew Patton

Jonathan Cohn

Michele Susan Yarnall

Sivamani Elumalai

Shujie Dong

Joshua Zvi Levin

Wenling Wang

Yuejin Sun

Martha M Dunn

David Nicholl

Nan Zhou

Lucy Qin

Jeff Bottoms

Mark Rose

Janet Louise Suttie

Michael Schweiner

Anic Deframond

Eric Levy

Yingping Lucy Qin

Joshua Levin

Mary-Dell Chilton

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross. The disclosure is also directed to methods of testing an edited haploid plant progeny for the presence of a first plant's genomic material.

Simultaneous gene editing and haploid induction

The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.

Methods and compositions for targeted genomic insertion

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Targeted insertion sites in the maize genome

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

Provided herein are promoters and related compositions and methods of use. Such promoters are useful for expression cassettes for plants, such as soybean. Such expression cassettes are useful, e.g., to drive expression of trait genes in plants, such as soybean. In some aspects, the disclosure provides an expression cassette comprising a promoter, a 5' UTR, and an intron.

Soybean promoters and uses thereof

Provided herein are regulatory elements, such as promoters and terminators, derived or obtained from variousspecies. Such regulatory elements are useful for expression cassettes for plants, such as soybean. Such expression cassettes generally contain a promoter and terminator sequence to control expression of each coding sequence. Provided are promoters and terminators which are useful for expressing insect resistance and herbicide tolerance genes.

Glycine regulatory elements and uses thereof

The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications

The invention provides three novel disarmed strains ofbacteria useful for the transformation of plants. The invention provides three engineeredChry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

strains

The present invention is drawn to compositions and methods for improving transformation frequency. The compositions, synthetic selectable marker genes, are used in transformation methods and result in increased transformation frequency.

Methods for improving transformation frequency

A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

Corn event MIR162

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Research Triangle Park, NC, United States of America

Qiudeng Que