E.i. Dupont De Nemours and Company

Nutrition & Biosciences USA 4, Inc.

Dupont Industrial Biosciences Usa, LLC

E. I. Du Pont Nemours and Co.

Qiong Cheng

Luan Tao

Robert DiCosimo

Mark Scott Payne

Pierre E Rouviere

Zheng You

Kristin Ruebling-Jass

Jayme L Paullin

Rakesh Nambiar

Jian Ping Lai

Stephen R Fahnestock

Yougen Li

Ellen D Semke

Jahnavi Chandra Prasad

Kristy N Kostichka

Vasantha Nagarajan

Tanja Maria Gruber

Stuart M Thomas

Natalia Sedkova

Qi Chen

Richard Ray Bott

Susan Marie Hennessey

Jean-Francois Tomb

Arthur Ouwehand

Jared B Parker

Veli Alkan

Hong Peng Wang

Michael G Bramucci

Stephen K Picataggio

Yefim Brun

James M Odom

Steven Cary Rothman

Zhenghong Zhang

Yuanfeng Liang

Mattheos Koffas

Kelley C Norton

Rong Guan

Charles R Powley

Kevin Michael Croker

Hongxian He

Slavko Kralj

Albert W Alsop

Mario W Chen

Xiao-Song Tang

Wonchul Suh

Katharine Janet Gibson

Linda Jane DeCarolis

Patricia C Brzostowicz

Linda Jane Solomon

Andreas Schenzle

Lisa Diane Reiss

Joanne Y Shyr

Jianzhong Zhang

Andrew C Eliot

Zheyong Yu

Michael P Perry

Pamela L Sharpe

Annapurna Sachan

Melissa D Bosak

Thomas Eduard Friedmann

Henry Yao

Ritu Bhalla

Deana DiCosimo

Edward S Miller

Neeraj Pandey

Pierre E Rouvier

Kristy Nan-Shan Kostichka

Robert Dicosimo

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Engineered glucosyltransferases

Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.

Enzymatically polymerized gelling dextrans

An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

Enzymatic synthesis of soluble glucan fiber

Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.

Polypeptides capable of producing glucans having alpha (1-->2) linkages and use of the same

An enzymatically produced α-glucan oligomer/polymer compositions is provided. The enzymatically produced α-glucan oligomer/polymers can be derivatized into α-glucan ether compounds. The α-glucan oligomers/polymers and the corresponding α-glucan ethers are cellulose and/or protease resistant, making them suitable for use in fabric care and laundry care applications. Methods for the production and use of the present compositions are also provided.

Glucan fiber compositions for use in laundry care and fabric care

An enzymatically produced &#x3b1;-glucan oligomer/polymer compositions is provided. The enzymatically produced &#x3b1;-glucan oligomer/polymers can be derivatized into &#x3b1;-glucan ether compounds. The &#x3b1;-glucan oligomers/polymers and the corresponding &#x3b1;-glucan ethers are cellulose and/or protease resistant, making them suitable for use in fabric care and laundry care applications. Methods for the production and use of the present compositions are also provided.

An enzymatically produced soluble &#x3b1;-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble &#x3b1;-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble &#x3b1;-glucan fiber are also provided.

Growing community of inventors

Wilmington, DE, United States of America

Qiong Cheng