Central Lab of Tianjin Academy of Agricultural Sciences

Qingkuo Lan

Xin Zhao

Yong Wang

Yi Cheng

Zhu Zhu

The invention discloses a method for detection of genetically modified maize BT11. The principle of the method is that the DNA template of the sample is amplified at a temperature of 63&#xb0; C.&#x2dc;65&#xb0; C. for 45&#x2dc;60 min by using 4 specific primers and a DNA polymerase with strand displacement activity. The identification thereof is to make a judgment on whether BT11 component is contained in the sample by directly observing the turbidity in the reaction tube or the color change after the addition of SYBR Green with naked eyes or by agarose gel electrophoresis. The detection method of the invention has the advantages of high specificity, quickness, simplicity and convenience and the like, which provides a convenient method for detection of genetically modified maize BT11 with an extensive application prospect.

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Tianjin, China

Qingkuo Lan