Genemind Biosciences Company Limited

Direct Genomics Co., Ltd.

Qin Yan

Zefei Jiang

Zhiliang Zhou

Huan Jin

Weibin Xu

Ping Wu

Linsen Li

Ruitao Sun

Luyang Zhao

Liangjin Ge

Lei Li

Jianfeng Xu

Fang Zhou Chen

Songzhen Zhang

Yuxiang Chen

Jie Jian

Jiahong Xu

Bin Hua Liu

Meng Zhang

Bin Liu

Guangming Wang

Jiao Zheng

Jianming Zeng

Jifang Qi

Qunting Lin

Gailing Li

Yunyang Cheng

Guangli Gan

Zeifei Jiang

The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.

Method for controlling base sequence determination, base sequence determination system and control device

Disclosed are a method for ligating nucleic acid fragments, a method for constructing a sequencing library, and the use thereof. The method for ligating nucleic acid fragments comprises ligating a first nucleic acid fragment and a second nucleic acid fragment in a mixed enzyme reaction system comprising a DNA ligase and a RNA ligase, wherein the first nucleic acid fragment is double-stranded DNA. The ligation efficiency of the method for ligating nucleic acid fragments can reach 85% or more, and by using this ligation method to construct a sequencing library, the yield of the effective library is significantly improved.

Method for ligating nucleic acid fragments, method for constructing sequencing library, and use

The present invention relates to a nucleoside analog having the structure of the following formula, a method for preparing the nucleoside analog, and an application of the nucleoside analog in nucleic acid sequencing, etc.

Nucleoside analogue, preparation method and application

A method and device for image registration, and a computer program product. The method includes performing, based on a reference image, a first registration of an image to be registered, including determining a first offset between predetermined areas on the image and the reference image, and moving all bright spots on the image based on the first offset; performing, based on the reference image, a second registration with respect to the first registered image, including merging the first registered image and the reference image, calculating the offset of all overlapped bright spots in the predetermined area on the merged image to determine a second offset, two or more bright spots distanced by less than a predetermined number of pixels being one overlapped bright spot, and moving, on the basis of the second offset, all bright spots on the first registered image, thus implementing the registration of the image.

Method and device for image registration, and computer program product

A nucleic acid loading device, including a housing, a carrying module, a motion module, a liquid circuit module and a control module. The motion module includes a support platform, first and second driving mechanisms, and a carrying member. The liquid circuit module is configured to control a reagent to flow into and out of a reactor. The control module is connected with the motion module and the liquid circuit module, and the control module is configured to control the first and second driving mechanisms, the liquid circuit module and the temperature control unit. A first face of a semiconductor cooler heats or refrigerates during operation, providing heat for an accommodating seat through a heat conducting body such that the reactor can be at different ambient temperatures. The cooperation between the motion and liquid circuit modules may achieve automation and industrialization of loading a sample into the reactor.

Carrying module, nucleic acid loading device and use thereof

An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.

Optical system, method for calibrating optical system, and sequencing system

A method and device for detecting bright spots on an image. The image is acquired from a field of view where a base extension reaction occurs, multiple nucleic acid molecules with optically detectable labels exist in the field of view where the base extension reaction occurs, and at least some of the nucleic acid molecules appear as bright spots on the image. The method includes preprocessing the image to obtain a preprocessed image; determining a critical value to simplify the preprocessed image to obtain a simplified image, determining a first bright spot detection threshold on the basis of the preprocessed image, and identifying candidate bright spots on the image on the basis of the preprocessed image and the simplified image, including determining a pixel matrix that satisfies at least two of conditions as a candidate bright spot.

Method and device for detecting bright spots on image, and computer program product

The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.

Imaging method, device and system

Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): 

The disclosure discloses an alignment method, device, and system. The alignment method includes: converting each read into a set of short fragments corresponding to the read to obtain a plurality of sets of short fragments; determining a corresponding position of the short fragment in a reference library to obtain a first positioning result, wherein the reference library is a hash table constructed based on a reference sequence, the reference library includes a plurality of entries, one entry of the reference library corresponds to one seed sequence, and the seed sequence is capable of matching at least one sequence on the reference sequence, a distance between two seed sequences corresponding to two adjacent entries of the reference library on the reference sequence is less than a length of the short fragment; removing a short fragment positioned on any one of the adjacent entries of the reference library in the first positioning result to obtain a second positioning result; and extending based on short fragments from the same read in the second positioning result to obtain an alignment result of the read. The alignment method can efficiently and accurately process and position sequencing data.

Alignment methods, devices and systems

Disclosed are a positioning method, a positioning apparatus and a sequencing system (). The sequencing system () comprises an imaging assembly () and a mobile platform (), wherein the imaging assembly () comprises an optical axis (OP), the mobile platform () bears a reaction apparatus (), the stroke of the mobile platform () in a first direction is controlled by means of a limiting switch, and the stroke comprises a first limit. The method comprises the steps: (a) moving, by a first step distance, the mobile platform () in a direction approaching the first limit, until the distance between a current position of the reaction apparatus () and the previous position of the reaction apparatus () is less than the first step distance; (b) moving the mobile platform () in a direction that is the opposite of the moving direction in step (a); (c) moving the mobile platform () in the moving direction in step (a), until the mobile platform () reaches the first limit; and (d) moving, by a preset distance, the mobile platform () in the direction that is the opposite of the moving direction in step (a), so that the positions of the optical axis (OP) and the reaction apparatus () satisfy a preset relationship. According to the method, the quick and precise positioning of the mobile platform () can be realized by means of moving back and forth in the first direction, such that the positions of the optical axis (OP) and the reaction apparatus () satisfy a preset relationship.

Positioning method, positioning apparatus and sequencing system

The present disclosure relates to base sequence determination. A base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit is defined as: a second biochemical reaction&#x2014;a first biochemical reaction&#x2014;photographing. A method includes, after initiation steps, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: using the fluid device to perform the first biochemical reaction of the sample on the first component, using the optical device to photograph the sample on the first component after the first biochemical reaction, and using the fluid device to perform the first biochemical reaction of the sample on the second component.

A method for constructing a sequencing template based on an image, a device, and a system. The image includes first, second, third and fourth images of one same field of view corresponding to base extensions of A, T/U, G, and C respectively; the first, second, third and fourth images respectively include images Mand M, images Nand N, images Pand P, and images Qand Q; the method includes combining any two of the images M, M, N, N, P, P, Q, and Qto perform bright spot matching, and enabling such images to participate in the combination for at least one time to obtain a plurality of combined images including first coincident bright spots, and merging the first coincident bright spots on the plurality of combined images to obtain a bright spot set corresponding to the sequencing template.

Method for constructing sequencing template based on image, and base recognition method and device

Disclosed is a chip. The chip comprises a substrate () and a base layer () in pressing arrangement with the substrate; the substrate comprises a first surface () and a second surface () in opposite arrangement, reaction tank arrays formed by a plurality of flowing channels () are arranged on the first surface of the substrate at intervals, two oppositely arranged side walls () of each flowing channel () stretch along the length direction of the flowing channel () and intersect at two ends of the flowing channel to form two tapered tail ends () with included angles, and a fluid inlet hole () and a fluid outlet hole () which are communicated with the second surface of the substrate are respectively provided on the surfaces of the two tapered tail ends (); and the base layer () comprises a transparent base () and a spacing layer () arranged on the surface of the transparent base, the spacing layer () is in contact with the firs surface () of the substrate, and a corrosion groove is provided on the spacing layer () corresponding to a position where the flowing channel () is located. The flow field distribution of the chip is good, the deformation rate of a base in the chip is low, and the fluid in the chip can be fully flushed or replaced. Also disclosed is an application of the chip.

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Shenzhen, China

Qin Yan