Syngenta Crop Protection Ag

China National Seed Group Corporation, Ltd.

Qin Wen

Chao Li

Chao Tan

Bolin Liu

Wanggen Zhang

Qianqian Yang

Jieting Xu

Chonglie Ma

Jianping Xu

Long Qiu

This disclosure relates to methods of transformation of dicots, such as tomato and watermelon. In some aspects, the methods comprise use of dicot explants obtained by dividing seeds and soaking the dicot explants in various media. In some aspects, transformed cells of a dicot plant are provided as well as dicot plants comprising transformed cells.

Methods for transformation of dicot plant cells

The present disclosure relates to a gene sequence Cry1Ab/Cry1AcZM, an expression cassette containing the gene, an expression vector, a host cell and the use thereof in plant breeding. The protein encoded by an insect-resistant gene according to the disclosure can be expressed in a monocotyledonous plant and further used for culturing an insect-resistant transgenic monocotyledonous plant harboring transgenes Cry1Ab/Cry1AcZM. Bioassay tests prove that the modified and synthesized gene sequence Cry1Ab/Cry1AcZM and the expression product of the constructed vector according to the disclosure have a killing effect on lepidopteran pests.

Gene for encodingcrystal proteins, and use thereof

Growing community of inventors

Beijing, China

Qin Wen