Huazhong Agricultural University

Shenzhen Institute of Molecular Crop Design

China National Seed Group Co., Ltd.

Frontier Laboratories of Systems Crop Design Co., Ltd.

Qifa Zhang

Wei Chen

Fasong Zhou

Jing Li

Junli Zhou

Hang He

Xingwang Deng

Xiaoyan Tang

Haodong Chen

Weibo Xie

Huihui Yu

Yuqing He

Yongzhong Xing

Weiya Xue

Chuchuan Fan

The present invention provides a rice whole genome breeding chip and the application thereof. The rice whole genome breeding chip of the present invention is Rice60K, an SNP chip manufactured based on Infinium technique. Each chip can detect 24 samples simultaneously and contains 58,290 SNP sites. The marker sites have DNA sequences represented by SEQ ID NO.1-58290. The chip can be used in molecular marker fingerprint analysis of the rice variety resources, in genotype identification of the hybrid progeny population, in identification of the variety authenticity, in analysis and screening of the genetic background of the breeding materials, and in association analysis of the agronomic traits, having wide application prospects.

Rice whole genome breeding chip and application thereof

The cloning and application of a pleiotropic gene Ghd7 that controls grains yield, heading date and plant height of rice are provided. The gene has the nucleotide sequence as shown in SEQ ID NO: 1. The sequence of the present gene is 3,917 bp in length, contains two exons and encodes 257 amino acids. The cDNA sequence of the gene is as shown in SEQ ID NO: 1. The present gene encodes a protein having the CCT domain of CO protein and having the amino acid sequence as shown in SEQ ID NO: 1. Rice plants transformed with GHD7 gene are obtained using transgenic technology. The transgenic plants all exhibit markedly increased yield, larger number of spikelets per panicle, delayed heading date and elevated plant height as compared to their respective controls (wild type receptors plants). The trait changes are quite consistent with the phenotypes of the two parent genotypes of GHD7 near isogenic lines of Zhenshan 97. The methods for gene cloning, breeding of near isogenic lines and transgene manipulation are also provided, as well as the application of pleiotropic gene GHD7 in research of rice breeding and plant evolution.

Cloning and application of a pleiotropic gene Ghd7 that controls grains yield, heading date and plant height of rice

Growing community of inventors

Wuhan, China

Qifa Zhang