Mgi Tech Co., Ltd.

Aac Technologies Pte. Ltd.

Qiaoling Li

Radoje Drmanac

Hui Fu Jiang

Wenwei Zhang

Yuan Jiang

Xia Zhao

Bo Wang

Li Chen

Shengmao Liu

Bo Xiao

Dong Li

Jinquan Huang

Andrei Alexeev

Tong Wang

Chao Dong

Evan Hurowitz

Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element

A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is provided on primer sequences and a nicking enzyme recognition sequence is either provided or not provided on same, and a first affinity tag is provided on one of the primer sequences; using USER enzyme to cleave the first product; cyclizing the cleaved first product; treating the cyclization product with either a phosphatase or a nicking enzyme; using a solid-phase vector for combination with a cyclized molecule; performing a restrictive gap translation reaction; removing by digestion any portion that did not undergo the restrictive gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and cyclizing a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments, a simplified library construction process, reduced library construction time, and reduced library construction costs.

Method and reagent for constructing nucleic acid double-linker single-strand cyclical library

The present disclosure discloses a speaker including a frame with a receiving space; a conductive element assembled with the frame; and a vibration system positioned by the frame, including a diaphragm received in the receiving space and a voice coil driving the diaphragm. The diaphragm divides the receiving space into a front cavity above the diaphragm and a rear cavity below the diaphragm. The frame includes an upper surface facing the diaphragm and an assemble surface opposed to the upper surface, and the conductive element includes a first conducting part disposed out of the assemble surface for electrically connecting to the voice coil.

Speaker

Growing community of inventors

Shenzhen, China

Qiaoling Li