Chinese Academy of Medical Sciences

Genetron Health (beijing) Co., Ltd.

Qianqian Song

Pei Wang

Kun Chen

Sizhen Wang

Yuchen Jiao

Hai Yan

Yuting Wang

Chunfeng Qu

Hui Liu

The present invention provides a kit for early screening of hepatocellular carcinoma, comprising a gene marker detection reagent and a protein marker detection reagent. The invention also provides a preparation method and application of the kit. The kit comprising specific gene markers and protein markers of the present invention has been demonstrated to be effective in achieving early screening of HCC in community populations, particularly in prospective studies.

Kit for early screening of liver cell cancer and preparation method and use thereof

A method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer. The library construction method and sequencing data analysis process have the following advantages: 1. Simultaneous detection of multiple mutation forms in liver cancer without capturing; 2. Suitable for efficient capture of ultra-small target regions; 3. The library may support 10-20 tests; 4. Ligate the DNA barcode to the starting ctDNA molecule during the library construction process, and cooperate with the biological information analysis process to achieve high specific detection of low-frequency mutations in ctDNA; 5. The library is usable for PCR hot spots detection and sequencing by a capture method at the same time, the added DNA barcode may effectively filter out false positive mutations and achieve high-specificity sequencing based on duplex. The disclosure helps with early screening, disease tracking, efficacy evaluation, prognosis prediction and the like of liver cancer.

Method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer

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Beijing, China

Qianqian Song