Liangshan Branch of Sichuan Tobacco

Sichuan Branch of China Tobacco

Chinese Academy of Agricultural Sciences

Tobacco Resarch Inst. of Chi. Acad. of Agr. Sci

Qiang Lei

Xiang Xu

Bin Li

Lili Shen

Changchun Feng

Ying Li

Dongyang Liu

Yong Wang

Fenglong Wang

Jinguang Yang

Liyun Song

Lianqiang Jiang

The invention belongs to the field of genetic engineering technology and application thereof, and in order to solve the problems of lack of high efficient TMV-resistant RNAi nano-preparation at present, complicated preparation process of medicament, poor stability and delivery efficiency of dsRNA, the invention provides an RNAi nano-preparation, preparation method thereof and application thereof in TMV prevention and control. The RNAi nano-preparation is prepared from dsRNA and chitosan nano materials, wherein, dsRNA is a highly TMV-resistant RdRP3 gene with a length of 313 bp obtained from screening, with nucleotide sequence shown in SEQ ID NO.1, and the volume ratio of the chitosan to 1 &#x3bc;g/&#x3bc;l dsRNA is 10:(1-6). The RNAi nano-preparation provided by the invention has the advantages of stronger stability and better durability of dsRNA, good biocompatibility, good biodegradability, no harm to crops, environmental friendliness and the like, and has a good application prospect in the field of TMV virus prevention and control.

RNAi nano-preparation, preparation method thereof and application thereof in TMV prevention and control

The present invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel use of prodigiosin in resisting potato virus Y. Application of prodigiosin in preparing a medicine for preventing and controlling potato virus Y. The medicine for preventing and controlling the potyvirus is a liquid or solid preparation containing prodigiosin. It is clarified that PVY promotes its own replication and infection by recruiting host factor Hsp70 in the process of infecting the host, realizing virus infection and spread. Hsp70 plays an important role in the infection and replication of plant viruses. Based on the above results, it is further clarified that prodigiosin treatment can significantly increase the ubiquitination level of the plant host, promote the ubiquitination of HSP70 protein of the host, degrade or inhibit the expression of Hsp70 protein, activate the natural immune activity of the host plant.

Use of prodigiosin in resisting potyvirus

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Chengdu, China

Qiang Lei