Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Oilcrops Research Institute of Chinese Acadamy of Agriculture Sciences

Wuhan Institute of Technology

Oil Crops Research Institute, Chinese Acadamy of Agricultural Sciences

Qi Zhang

Peiwu Li

Wen Zhang

Xiaoxia Ding

Zhaowei Zhang

Jun Jiang

Xiaoqian Tang

Du Wang

Liangxiao Zhang

Xuefang Wang

Min Li

Xiupin Wang

Ting He

Yizhen Bai

Zhe Yuan

Huali Xie

Hui Li

Wei Lv

Xiaofeng Hu

Xin Li

Jing Liu

Tianyi Liu

Fei Ma

Yanru Wang

Ran Li

Longlong Ma

Jianbo Wan

Tong Wang

Xiaofeng Yue

Jinxin Xu

Shengwei Shi

Chenguang Wang

Yuting Zhu

The present disclosure discloses a method for recycling a residue from MXene preparation, including the following steps: recovering a bottom residual sediment produced in preparation of MXene through etching in a minimally intensive layer delamination (MILD) method, mixing the bottom residual sediment with a molten polyvinyl alcohol (PVA) solution, and drying to prepare a TiCT-TiAlC/PVA composite film. The present disclosure can effectively utilize a residue from an MXene process to prepare a composite film with both excellent mechanical properties and electrical conductivity. The composite film has extremely-high sensitivity for stress-strain and prominent stability, and is suitable for flexible connection and sensing of biosensors, robots, or the like. The present disclosure has significant economic and environmental benefits, and is suitable for promotion and application.

Method for recycling residue from MXene preparation and use of residue in biosensor

A method for identifying and evaluating toxigenic capability of an aflatoxigenic strain. A ratio of the aflatoxin yield to Nor-1 gene transcriptional quantity is determined to have high relative stability. Anstrain toxigenic capability identification model is established, and thus a regression equation between thetoxigenic capability and the ratio AFT/Nor-1 of the aflatoxin yield to the Nor-1 gene transcriptional quantity is obtained.

Method for identifying and evaluating toxigenic capability of aflatoxigenic strain

A time-resolved fluorescence immunochromatography kit for the simultaneous detection of a mixed pollutant of aflatoxin and carbaryl, a preparation method therefor, and an application thereof. The kit comprises a time-resolved fluorescence immunochromatography test strip and a sample reaction vial containing a europium-labeled anti-aflatoxin monoclonal antibody and a europium-labeled anti-carbaryl monoclonal antibody lyophilized product, detection lines of the time-resolved fluorescence immunochromatography test strip being coated with an aflatoxin-bovine serum albumin conjugate and a carbaryl-ovalbumin conjugate respectively, and the anti-carbaryl monoclonal antibody is produced by secretion of a hybridoma cell line Jnw1D2 with a preservation number of CCTCC NO. C201654. The kit can be used for the simultaneous detection of aflatoxin and carbaryl content in a sample, and features simple and quick operations and a high sensitivity.

Time-resolved fluorescence immunochromatography kit for simultaneous detection of mixed pollutant of aflatoxin and carbaryl, preparation method therefor, and application thereof

A time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and an application thereof are disclosed. The kit comprises a time-resolved fluorescent immunochromatographic test strip and sample reaction vials each containing a europium-labeled monoclonal antibody lyophilized product; wherein the fluorescent test strip comprises a PVC substrate, and a surface of the PVC substrate is adhered with a water absorbing pad (), a detection pad () and a sample pad () from top to bottom, adjacent pads being connected and overlapping at connections. The detection pad () adopts a nitrocellulose membrane as the base thereof and is arranged with a lateral quality control line () and five detection lines () from top to bottom each covered by a bovine serum albumin conjugate of each toxin. The fumonisin B1 monoclonal antibody is generated by the hybridoma cell strain Fm7A11 having China Center for Type Culture Collection (CCTCC) accession number C201636. The kit is applicable to simultaneous detection of mixed contamination of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and sterigmatocystin.

Time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and application thereof

The present invention relates to an early warning method before the occurrence of aflatoxin contamination. The steps are as follows: extracting toxins from the sample to obtain a sample extract, and subjecting the sample extract to detection and analysis by liquid chromatography-high resolution mass spectrometer, performing qualitative analysis based on the mass spectrometry information to obtain qualitative results, performing quantitative analysis based on a standard curve of the chromatographic peak area of each warning molecule/the peak area of the internal standard-warning molecule concentration to obtain quantitative results of these warning molecules, wherein a risk of aflatoxin contamination of the sample is assessed to obtain a classification prediction model, inputting the quantitative results of the warning molecules for a toxigenic strain of, and outputting a risk assessment result based on the classification prediction model, thereby achieving the early warning before aflatoxin contamination occurs.

Early warning method before occurrence of aflatoxin contamination

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate. The three mycotoxins including 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin can be rapidly and synchronously detected.

Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1, and sterigmatocystin

A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol, and T-2 toxin

An immunochromatography test strip for detecting pollution of diacetoxyscirpenol includes a bottom plate, where a water absorption pad, a detection pad, a gold-labeled pad and a sample pad are sequentially attached to one side of the bottom plate from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane, the quality control line is coated with a rabbit antimouse polyclonal antibody, the detection line is located below the quality control line, and the detection line is coated with a diacetoxyscirpenol-ovalbumin conjugate; the gold-labeled pad is transversely spray-coated with a nanogold-labeled anti-diacetoxyscirpenol monoclonal antibody; and the anti-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO.C201881.

Immunochromatography test strip for detecting pollution of diacetoxyscirpenol, preparation method therefor, and application thereof

The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.

Myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and application thereof

The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.

Pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus

The present invention relates to the microbiological field, and particularly relates to aadcarboxglata biocontrol strain efficiently inhibiting production of aflatoxins by. Theadcarboxglata biocontrol strain Wt16 was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017331. Theadcarboxglata strain Wt16 is isolated from peanut pods for the first time which can significantly inhibit aflatoxins production by, and also has an extremely good effect on inhibiting aflatoxins production byfor peanuts from different sources.

biocontrol strain efficiently inhibiting production of aflatoxins byand application thereof

The invention discloses a NIR method for fatty acid content of oilseeds, including: selection of oilseed samples, and analyzing the oilseed samples by an near infrared spectrometer to obtain NIR spectra; preprocessing of NIR spectra and establishment of a NIR spectral database of oilseeds; establishment of a fatty acid database of oilseeds based on gas chromatography; establishment of prediction model of fatty acids in oilseeds; acquiring NIR spectrum of a sample to be tested by the near infrared spectrometer, and importing the preprocessed NIR spectrum into the prediction model of fatty acids to obtain the predicted fatty acid content of the tested sample. The method is simple and rapid to operate and is non-destructive, and the detection time of the sample is greatly shortened and the detection cost is reduced.

NIR spectroscopy method for fatty acid content of oilseeds

A method for multivariate adulteration detection on an edible oil includes (1) construction of a model: S1, acquiring near-infrared spectra of edible oils; S2, establishing a near-infrared spectral database of the edible oils; S3, establishing a multivariate adulteration detection model for a type of edible oil; and (2) application of the model: acquiring spectra of a sample to be tested according to the near-infrared spectral signal acquisition method in step S1, preprocessing the obtained near-infrared spectra by using the method in step S2 to obtain near-infrared spectral data of the sample, and determining the authenticity of the sample to be tested by using the multivariate adulteration detection model for the edible oil established in step S3. The method is simple and rapid in operation, can effectively and rapidly screen the authenticity of an edible vegetable oil, and has strong practicability.

Method for detecting multivariate adulteration of edible oil based on near-infrared spectroscopy

The present invention relates to a time-resolved fluorescent immunochromatographic assay rapid test kit for quantitative determination of the level of type I pyrethroid, which has the advantages of simple operation, rapid detection, and with high accuracy. The kit has a quantitative detection limit of 9.12 ng/mL for a type I pyrethroid sample. When the kit is applied to the detection of an actual sample of type I pyrethroid and by comparing the detection result obtained by HPLC, the kit has a coefficient of correlation of 0.986 (R). Hence, the kit is applicable to the detection by using the actual samples of type I pyrethroid.

Time-resolved fluorescent immunochromatographic assay rapid test kit for type I pyrethroid

An immunoadsorbent for purifying fumonisin B, aflatoxin B, ochratoxin A, zearalenone and sterigmatocystin; a complex affinity column and its preparation method; and a method for detecting the mycotoxins using the same are provided. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin Bmonoclonal antibody, an anti-aflatoxin Bmonoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin Bmonoclonal antibody is secreted by a hybridoma cell strain Fm7A11. The complex affinity column can be used for purification and detection of a fumonisin Bsample, an aflatoxin Bsample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

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Hubei, China

Qi Zhang