Shanghai Ruiyu Biotech Co., Ltd.

Puwen Luo

Rui Chen

Haohan Xia

Yifei Wu

Haiqing Huang

Haibo Yan

Xiaojia Zhang

A method for laser distance measuring includes: emitting an incident ray α having an incident angle δ to a reflective surface of a laser reflecting mirror (), the incident ray α being reflected by the laser reflecting mirror () to generate a first reflected ray β, and the first reflected ray β irradiating an object to be measured (); capturing a second reflected ray θ and generating a laser image on a laser imaging plane (), the second reflected ray θ being generated by the reflective surface of the laser reflecting mirror () reflecting a return ray γ generated after the first reflected ray β irradiates a surface of the object to be measured; and determining, based on the laser image, a measurement distance A according to a geometrical trigonometry.

Methods and systems for laser distance measuring, fousing methods and systems, and devices for auto-focusing analysis

A method and a system for fluorescence intensity of a fluorescence image are provided. In the method, fluorescence imaging is performed on a target sample to obtain a fluorescent image. Edge extraction and segmentation is performed on each detection target in the fluorescence image, to obtain the fluorescent image area of each detection target in the fluorescence image. At least one of a cumulative gray-scale value, a maximum gray-scale value and an average gray-scale value of the fluorescence image region and a diameter value of a bright field image region of each detected target is calculated. Then, the flow clustering analysis is calculated based on at least one of the cumulative grayscale value, the maximum grayscale value, the average grayscale value, and the bright field diameter value.

Method and system for determining fluorescence intensity of fluorescence image

A fluorescence microscopic imaging method includes: after a to-be-detected sample plate is placed, lightening, according to experimental requirements, at least one monochromatic fluorescence excitation light source with a same color among multiple monochromatic fluorescence excitation light sources as a target light source, where monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source obliquely enters a preset detection region of the to-be-detected sample plate; collecting, at a side of the to-be-detected sample plate facing away from the target light source, fluorescence of particles within the preset detection region excited by irradiation of monochromatic fluorescence excitation light emitted by the target light source, and magnifying the preset detection region a preset number of times; filtering the excited fluorescence of the particles within the preset detection region; and acquiring a fluorescence image of the preset detection region.

Fluorescent microscopic imaging method and apparatus

A follow-up fixed focus system includes a fixed focus device, and the second end of the fixed focus device is in contact with a sample plate; when the sample plate has a small protrusion, the second end of the fixed focus device is pushed by the protrusion of the sample plate. When the sample plate has a small depression, the second end of the fixed focus device is in contact with the sample plate all the time under the action of self gravity of an observation device, so that the imaging distance between an object lens and the sample plate is kept unchanged.

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Shanghai, China

Puwen Luo