Life Technologies Corporation

Priya Balachandran

Craig Cummings

Olga Petrauskene

Robert Tebbs

Paolo Vatta

Lily Wong

Patrick Zoder

Payman Fatemi

Arlene Nunez

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of severalspecies in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains offrom each other, for examplemay be detected independently of otherspp. Some embodiments also describe a duplexed assay that can detect(formerly-),, and. Kits for detection ofare also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN&#xae; assay. In some embodiments, 0.2-2 cfu ofspp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

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Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of, subspecies, serovar() in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detectwithout cross-reacting withC or other closely relatedspecies. In some embodiments, methods and kits of the disclosure may comprise a TaqMan&#xae; assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of severalspecies in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains offrom each other, for examplemay be detected independently of otherspp. Some embodiments also describe a duplexed assay that can detect(formerly incertae-sedis),, and. Kits for detection ofare also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN&#xae; assay. In some embodiments, 0.2-2 cfu ofspp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

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Foster City, CA, United States of America

Priya Balachandran