Enzo Life Sciences, Inc.

Enzo Biochem, Inc.

Enzo Life Sciences, Inc. C/o Enzo Biochem, Inc.

Dai, Lijun

Patton, Wayne Forrest

Ludlam, Anthony

Praveen Pande

Wayne Forrest Patton

Elazar S Rabbani

Zaiguo Li

Yuejun Xiang

Jannis G Stavrianopoulos

Jack Coleman

Maciej Bernard Szczepanik

Irina Lebedeva

Natarajan Raju

Dakai Liu

Wei Cheng

Rajesh Khazanchi

Joshua Rabbani

Mykhaylo Losytskyy

Anatoliy Balanda

Kateryna Volkova

Lijun Dai

Hilary J Cox

Vladyslava Kovalska

Sergiy M Yarmoluk

James J Donegan

Anthony Ludlum

Christian Merz

Anthony Ludlam

Dee Shen

Irina V Lebedeva

Elazar Rabbani

Provided is a compound comprising the structure:(SIG)-(SI-MOD). In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.

Self-immolative probes for enzyme activity detection

The invention provides compositions and methods for preparing high-quality labeled DNA for use in array-based comparative genomic hybridization in amounts sufficient therefor starting with a low amount of input genomic DNA, such as 50-250 nanograms input genomic DNA.

DNA labeling compositions and methods for comparative genomic hybridization

Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

Fluorescent dyes and methods of use thereof

Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.

Assays for detecting modified compounds

Provided is a compound having the structure:

Provided are compounds comprising two DNA supramolecular binding molecules covalently joined by a linker group. Also provided are multisignal labeling reagents comprising (i) an oligomer of nucleotides or nucleotide analogs; (ii) a DNA supramolecular binding molecule noncovalently bound to the oligomer; and (iii) a first reactive group or a first partner of a first binding pair covalently bound to the oligomer. Additionally provided are methods of producing multisignal labeling reagents.

Multisignal labeling reagents and processes and uses therefor

The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.

Monoazo dyes with cyclic amine as fluorescence quenchers

Provided is a compound having the structure:(SIG)-(SI-MOD)where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.

The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4'-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.

Methods and intermediates for synthesizing SK1-I

Monoazo dyes with cyclic amine as fluorescence quencers

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Holbrook, NY, United States of America

Praveen Pande