Oxford Nanopore Technologies Limited

Vib Vzw

Vrije Universiteit Brussel

Pratik Raj Singh

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Richard George Hambley

Han Karel Remaut

Michael Robert Jordan

Mark Bruce

Stephen Robert Young

Luke McNeill

Neil Roger Wood

Ramiz Iqbal Nathani

John Joseph Kilgour

Sander Egbert Van Der Verren

Nani Van Gerven

Han Remaut

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.

Mutant pore

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

Protein pores

Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.

Transmembrane pore consisting of two CsgG pores

Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.

Modified nanopores, compositions comprising the same, and uses thereof

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Oxford, United Kingdom

Pratik Raj Singh