Alexion Pharmaceuticals, Inc.

Pratik Jaluria

Hunter F Malanson

Rachael Alford

Siguang Sui

Provided herein are methods shifting the isoelectric profile of a recombinant protein product and the use of such methods in the manufacture of recombinant protein products. Also provided are recombinant protein products produced by such methods.

Methods of shifting an isoelectric profile of a protein product and uses thereof

A method for producing a recombinant polypeptide, comprising: (a) providing a 100 L to 25,000 L fed-batch bioreactor comprising (i) cells capable of expressing the recombinant polypeptide asfotase alfa (SEQ ID NO: 1), and (ii) a culture medium suitable for conducting such expression, the culture medium comprising about 25 &#x3bc;M to about 300 &#x3bc;M zinc; (b) culturing the cells under conditions suitable to express the recombinant asfotase alfa wherein the pH of the culture medium is about 6.7 to about 7.1, and wherein zinc is added into said culture medium such that the zinc concentration in the culture medium is maintained at a concentration of about 25 &#x3bc;M to about 300 &#x3bc;M of zinc.

Manufacturing of alkaline phosphatases

Provided herein are methods of replicating a large scale eculizumab production cell culture in a small scale culture.

Growing community of inventors

Madison, CT, United States of America

Pratik Jaluria